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Our Abpromise guarantee covers the use of ab24838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ELISA||Use at an assay dependent concentration.|
|Purification||Use at an assay dependent concentration.|
ab24838 staining human HeLa cells by ICC/IF. Cells were PFA fixed and blocked with 3% BSA for 1 hour at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. A Cy2® conjugated donkey anti-mouse antibody, diluted 1/300 was used as the secondary.
The slide was stained with Dapi, S Tag-Cy2 and CREST-Cy3.