Anti-S tag 抗体 (ab183674)
Key features and details
- Rabbit polyclonal to S tag
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-S tag antibody
S tag 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to S tag -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- S tag transfected 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183674の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000.
|
|
IP |
Use at an assay dependent concentration.
Use 2.5µg. |
特記事項 |
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WB
1/1000 - 1/10000. |
IP
Use at an assay dependent concentration. Use 2.5µg. |
ターゲット情報
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関連性
S tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. -
別名
- KETAAAKFERQHMDS tag antibody antibody
- S peptide antibody
- S peptide epitope tag antibody
画像
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All lanes : Anti-S tag antibody (ab183674) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : S tag transfected 293T whole cell lysate
Lysates/proteins at 30 µg per lane.12% SDS-PAGE
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S tag antibody ab183674 immunoprecipitates S tag protein in IP experiments. IP Sample: 650 µg whole cell lysate of S tag protein expressing HeLa cells (1), 20 μg whole cell lysate of transfected HeLa cells (2), Control with 2.5 μg of preimmune rabbit IgG (3). Immunoprecipitation of S tag protein by 2.5 μg of ab183674. 12% SDS-PAGE. The immunoprecipitated S tag protein was detected by ab183674 diluted at 1/10000. Anti-rabbit IgG was used as a secondary reagent.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab183674 は 4 報の論文で使用されています。
- Volkmar N et al. Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2. EMBO J 41:e110777 (2022). PubMed: 35993436
- Salari S et al. Evaluation of a new live recombinant vaccine against cutaneous leishmaniasis in BALB/c mice. Parasit Vectors 13:415 (2020). PubMed: 32787908
- Li C et al. A SnRK1-ZmRFWD3-Opaque2 Signaling Axis Regulates Diurnal Nitrogen Accumulation in Maize Seeds. Plant Cell 32:2823-2841 (2020). PubMed: 32699171
- Yin Z et al. Histone acetyltransferase MoHat1 acetylates autophagy-related proteins MoAtg3 and MoAtg9 to orchestrate functional appressorium formation and pathogenicity in Magnaporthe oryzae. Autophagy 15:1234-1257 (2019). PubMed: 30776962