S. pombe Histone H2A (phospho S129) peptide (ab17576)

製品の概要

  • 製品名S. pombe Histone H2A (phospho S129) peptide

製品の詳細

  • 由来Synthetic
  • アミノ酸配列
    • アクセッション番号P04910
    • 生物種Schizosaccharomyces pombe
    • 修飾phospho S129

特性

Our Abpromise guarantee covers the use of ab17576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking - Blocking peptide for Anti-Histone H2A (yeast) (phospho S129) antibody (ab17353)

  • 製品の状態Liquid
  • 備考

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

関連情報

  • 別名
    • H2A1
    • H2A2
    • Histone H2A-alpha
    • Histone H2A-beta
    • Histone H2A.1
    • Histone H2A.2
    • Hta 1
    • HTA 2
    • Hta1
    • Hta1p
    • HTA2
    • Hta2p
    • SPT 11
    • SPT11
    see all
  • 関連性Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Post-translational modification for Saccharomyces cerevisiae: Phosphorylated to form H2AS128ph (gamma-H2A) in response to DNA double-strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks. Phosphorylation is dependent on the DNA damage checkpoint kinases MEC1/ATR and TEL1/ATM, spreads on either side of a detected DSB site and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Gamma-H2A interacts with ARP4, a shared component of the NuA4 histone acetyltransferase complex and the INO80 and SWR1 chromatin remodeling complexes, and serves to recruit first NuA4, mediating histone H4 acetylation, and subsequently the INO80/SWR1 complexes, facilitating DNA resection, to DSB sites. Gamma-H2A is required for sequestering cohesin around the break site, which is important for efficient post-replicative double-strand break repair by homologous recombination, holding the damaged chromatid close to its undamaged sister template. Gamma-H2A is removed from the DNA prior to the strand invasion-primer extension step of the repair process and subsequently dephosphorylated by PPH3, a component of the histone H2A phosphatase complex (HTP-C). Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. N-acetylated by NAT4. Acetylated by ESA1, a component of the NuA4 histone acetyltransferase (HAT) complex, to form H2AK4ac and H2AK7ac. Glutamine methylation at Gln-106 (H2AQ105me) by NOP1 is specifically dedicated to polymerase I. It is present at 35S ribosomal DNA locus and impairs binding of the FACT complex. Sumoylated to from H2AK126su. May lead to transcriptional repression. Post-translational modification for Schizosaccharomyces pombe: Phosphorylated to form H2AS128ph (gamma-H2A) in response to DNA double-strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks. Phosphorylation is dependent on the DNA damage checkpoint kinases rad3/ATR and tel1/ATM, spreads on either side of a detected DSB site and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Gamma-H2A is required for recruiting crb2, a modulator of DNA damage checkpoint signaling, to DSB sites. Gamma-H2A is removed from the DNA prior to the strand invasion-primer extension step of the repair process and subsequently dephosphorylated. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. Acetylated by esa1 to form H2AK4ac and H2AK7ac.
  • 細胞内局在Nuclear

S. pombe Histone H2A (phospho S129) peptide 画像

  • All lanes : Anti-Histone H2A (yeast) (phospho S129) antibody (ab17353) at 1 µg/ml

    Lane 1 : S. pombe lysate (0.1 ug)
    Lane 2 : S. pombe lysate (0.1 ug) with S. pombe Histone H2A (phospho S129) peptide (ab17576) at 1 µg/ml
    Lane 3 : S. pombe lysate (0.1 ug) with S. pombe Histone H2A (unmodified ) peptide (ab17577) at 1 µg/ml


    Predicted band size : 15.5 kDa

S. pombe Histone H2A (phospho S129) peptide (ab17576) 使用論文

ab17576 has not yet been referenced specifically in any publications.

Product Wall

Application Other

Review text: I used this product to do a dot blot specificity test for cross reactivity of modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.

No primary antibody directed towards H2AS129phos was used in this dot blot assay. This peptide was chosen to ensure there was no cross reactivity between the antibodies we are using on a regular basis. We have yet to see any cross reactivity with this peptide using 12+ antibodies directed towards histone H3 modified antibodies (some from Abcam some not).
Sample: Human Purified protein

Primary antibody (in addition to 'Histone H2A (yeast) peptide - phospho S129')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used
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投稿 Nov 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"