製品の概要

  • 製品名
    Anti-S-nitrosocysteine antibody [HY8E12]
  • 製品の詳細
    Mouse monoclonal [HY8E12] to S-nitrosocysteine
  • 特異性
    ab94930 targets conjugated S-nitrosocysteine, it does not recognize free S-nitrosocysteine.
  • アプリケーション
    適用あり: ICC, WB, IHC-P, IHC-Frmore details
  • 免疫原

    Chemical/ Small Molecule corresponding to S-nitrosocysteine conjugated to Bovine Serum Albumin (BSA).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/1000 - 1/5000.

ab131369 can be used as blocking peptide.

WB 1/1000 - 1/2000.
IHC-P 1/1000 - 1/5000.

Fixation of tissue for use with this antibody should be done with glutaraldehyde. The use of paraformaldehyde in conjunction with glutaraldehyde may improve staining results.

ab131369 can be used as blocking peptide.

IHC-Fr Use at an assay dependent concentration.

ab131369 can be used as blocking peptide.

ターゲット情報

  • 関連性
    All mammalian cells contain low levels of nitrosylated proteins, that are thought to be regulated by Snitrosylation and denitrosylation. S nitrosylation of proteins serves as a ubiquitous post-translational modification that dynamically regulates a broad functional spectrum of proteins. The majority of these proteins are regulated by S nitrosylation on a single critical cysteine residue within an acidic/basic or hydrophobic structural motif, that may also be subject to oxygen or glutathione dependent modification, suggesting that S nitrosylation is a prototypic redox signal.

画像

  • Image from Ding SY et al., J Biol Chem. 2011 May 13;286(19):16768-74. Epub 2011 Mar 25.; Fig 2.; doi: 10.1074/jbc.M110.192799; May 13, 2011 The Journal of Biological Chemistry, 286, 16768-16774.

    Mouse pancreatic islets were treated with 30 nm GLP-1 for 10 min prior to lysis. GCK was immunoprecipitated and assessed for S-nitrosocysteine by Western blotting using ab94930.

参考文献

This product has been referenced in:
  • Bahnson ES  et al. Targeted Nitric Oxide Delivery by Supramolecular Nanofibers for the Prevention of Restenosis After Arterial Injury. Antioxid Redox Signal 24:401-18 (2016). Read more (PubMed: 26593400) »
  • Choi MS  et al. S-nitrosylation of fatty acid synthase regulates its activity through dimerization. J Lipid Res 57:607-15 (2016). Read more (PubMed: 26851298) »

See all 3 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount
30 µg
Specification
Melanoma cancer
Treatment
5gy Irradiation
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

投稿 Feb 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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