Anti-S-nitrosocysteine 抗体 [HY8E12] (ab94930)


  • 製品名Anti-S-nitrosocysteine antibody [HY8E12]
  • 製品の詳細
    Mouse monoclonal [HY8E12] to S-nitrosocysteine
  • 特異性ab94930 targets conjugated S-nitrosocysteine, it does not recognize free S-nitrosocysteine.
  • アプリケーション適用あり: ICC, WB, IHC-P, IHC-Frmore details
  • 免疫原

    Chemical/ Small Molecule corresponding to S-nitrosocysteine conjugated to Bovine Serum Albumin (BSA).



Our Abpromise guarantee covers the use of ab94930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/1000 - 1/5000.

ab131369 can be used as blocking peptide.

WB 1/1000 - 1/2000.
IHC-P 1/1000 - 1/5000.

Fixation of tissue for use with this antibody should be done with glutaraldehyde. The use of paraformaldehyde in conjunction with glutaraldehyde may improve staining results.

ab131369 can be used as blocking peptide.

IHC-Fr Use at an assay dependent concentration.

ab131369 can be used as blocking peptide.


  • 関連性All mammalian cells contain low levels of nitrosylated proteins, that are thought to be regulated by Snitrosylation and denitrosylation. S nitrosylation of proteins serves as a ubiquitous post-translational modification that dynamically regulates a broad functional spectrum of proteins. The majority of these proteins are regulated by S nitrosylation on a single critical cysteine residue within an acidic/basic or hydrophobic structural motif, that may also be subject to oxygen or glutathione dependent modification, suggesting that S nitrosylation is a prototypic redox signal.

Anti-S-nitrosocysteine antibody [HY8E12] 画像

  • Image from Ding SY et al., J Biol Chem. 2011 May 13;286(19):16768-74. Epub 2011 Mar 25.; Fig 2.; doi: 10.1074/jbc.M110.192799; May 13, 2011 The Journal of Biological Chemistry, 286, 16768-16774.

    Mouse pancreatic islets were treated with 30 nm GLP-1 for 10 min prior to lysis. GCK was immunoprecipitated and assessed for S-nitrosocysteine by Western blotting using ab94930.

Anti-S-nitrosocysteine antibody [HY8E12] (ab94930) 使用論文

This product has been referenced in:
  • Ding SY  et al. Glucagon-like peptide 1 stimulates post-translational activation of glucokinase in pancreatic beta cells. J Biol Chem 286:16768-74 (2011). WB . Read more (PubMed: 21454584) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount 30 µg
Specification Melanoma cancer
Treatment 5gy Irradiation
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C

Ms. Seontae Kim

Verified customer

投稿 Feb 19 2013

Thank you for your email.
My offer is still active. You can choose any antibody as a replacement.
I have checked the immunogen sequence homology with the products we have in catalogue and the sequences you have kindly sent; unfortunately there ...

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Could you update me if you are still interested in ab94930?
Many thanks for your cooperation.

Thank you very much for your patience!
Once a vial is opened, we do not request back the same product. I suggest throwing the vial ab50185 away.
We now have peptide S-nitrosocysteine peptide available in catalogue. The catalogue number is ab1...

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Thank you very much for your cooperation.
I am sorry we are unable to determine the reason why this antibody isn't working. After reviewing the details you have provided the antibody should have worked however because same lysates are giving diffe...

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Thank you for your email. I am sorry to hear that you are experiencing problems with this antibody.
I have checked the details you have kindly provided and would like to ask few questions in order to understand the problems;
- Could you pleas...

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Thank you for contacting us. Yes, you are correct. The antibody should bind to all proteins that have the S-nitrosocysteine modification. I am not sure where the 1 kDa comes from, but this is incorrect and I have removed it from the datasheet. ...

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