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Synthetic peptide, corresponding to residues in Human RYK (intracellular domain).
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format. The following lots are still unpurified and still in stock as of 7th of March 2018- GR87562-9, GR87562-10 and GR87562-11. Lot numbers other than GR87562-9, GR87562-10 and GR87562-11 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab124961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/200000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
Purified ab124961 staining RYK in Jurkat (Human T cell leukemia T lymphocyte) cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody using purified anti-RYK, ab124961. An AlexaFluor®488 Goat anti-Rabbit was used as the secondary antibody. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), ab195889 was used as a counterstain antibody. DAPI was used as a nuclear counter stain. Confocal image showing nuclear and cytoplasmic staining in Jurkat cells.
Purified ab124961 staining RYK in Human ovarian carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehye- fixed, paraffin embedded sections). Tissue was fixed with paraffin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antbody at 1:400 dilution. A ready to use Goat Anti- Rabbit H&L (HRP). Hematoxylin was used as a counterstain. Cytoplasmic staining on human ovarian carcinoma.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure: Lane 1: 30 seconds
Lane 2: 10 seconds
Purified ab124961 staining RYK in Jurkat (Human T cell leukemia T lymphocyte) cell line by Flow Cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Samples were incubated with primary antibody at 1:40 dilution (red). An Alexa Fluor® 488 Goat anti-rabbit IgG , ab150077 was used as a secondary antibody at 1:2000 dilution. A rabbit monoclonal IgG, ab172730 was used as an isotype control (black). Cell without incubation with primary and secondary antibodies - unlabeled control (blue).
Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate, 10μg
Lane 2 (+): HEK-293 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124961 in HEK-293 whole cell lysate
Purified ab124961 immunoprecipitating in HEK-293 whole cell lysate. For western blotting, primary antibody used was purified ab124961 at 1:500 dilution. Ab131366 VeriBlot for IP (HRP) was used as the secondary antibody at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"