製品の概要

  • 製品名Anti-RYBP antibody
    RYBP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RYBP
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Monkey, Macaque Monkey, Gorilla, Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human RYBP.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; K562; A549; JEG3. This antibody gave a positive signal in the following cell lines: HeLa, HepG2, MCF-7 (Methanol fixed), and in HepG2 (Formaldehyde fixed).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab107896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. In tumor cell lines, may induce apoptosis.
  • 組織特異性Widely expressed with highest levels in lymphoid tissues and placenta.
  • 配列類似性Contains 1 RanBP2-type zinc finger.
  • 細胞内局在Nucleus. Cytoplasm. Primarily found in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2410018J24Rik antibody
    • AAP 1 antibody
    • AAP1 antibody
    • APAP 1 antibody
    • APAP-1 antibody
    • APAP1 antibody
    • Apoptin associating protein 1 antibody
    • Apoptin-associating protein 1 antibody
    • cb337 antibody
    • Death effector domain associated factor antibody
    • Death effector domain-associated factor antibody
    • Death effector domain-associated factor B antibody
    • DED associated factor antibody
    • DED-associated factor antibody
    • DED-associated factor B antibody
    • DEDAF antibody
    • MGC62213 antibody
    • RING1 and YY1 binding protein a antibody
    • RING1 and YY1 binding protein antibody
    • RING1 and YY1-binding protein antibody
    • RING1 and YY1-binding protein B antibody
    • Ring1 interactor RYBP antibody
    • RYBP antibody
    • RYBP_HUMAN antibody
    • rybpa antibody
    • wu:fi34f03 antibody
    • YEAF 1 antibody
    • YEAF1 antibody
    • YY1 and E4TF1 associated factor 1 antibody
    • YY1 and E4TF1-associated factor 1 antibody
    • YY1- and E4TF1/GABP-associated factor 1 antibody
    • zgc:153842 antibody
    see all

Anti-RYBP antibody 画像

  • All lanes : Anti-RYBP antibody (ab107896) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • ICC/IF image of ab107896 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107896 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa and HepG2 cells, and in 4% Formaldehyde fixed (10min) HepG2 cells at 1ug/ml.

Anti-RYBP antibody (ab107896) 使用論文

ab107896 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"