Anti-Ryanodine Receptor 抗体 [C3-33] (ab2827)

製品の概要

  • 製品名
    Anti-Ryanodine Receptor antibody [C3-33]
    Ryanodine Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [C3-33] to Ryanodine Receptor
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, ICC, Flow Cyt, IHC-P, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Chicken, Guinea pig, Dog, Human, Pig, Fish, Amphibian
    交差が予測される動物種: Horse
  • 免疫原

    Full length protein corresponding to Dog Ryanodine Receptor. Canine cardiac ryanodine receptor.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    from ascites
  • 一次抗体 備考
    The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
  • ポリ/モノ
    モノクローナル
  • クローン名
    C3-33
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use a concentration of 1 µg/ml.
ICC 1/50.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 µg/ml. Staining of RyR in rat cardiac tissue results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. This antibody detects a 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor.

ターゲット情報

  • 機能
    Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm. Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis.
  • 組織特異性
    Skeletal muscle and brain (cerebellum and hippocampus).
  • 関連疾患
    Malignant hyperthermia 1
    Central core disease of muscle
    Multiminicore disease with external ophthalmoplegia
    Myopathy, congenital, with fiber-type disproportion
    Defects in RYR1 may be a cause of Samaritan myopathy, a congenital myopathy with benign course. Patients display severe hypotonia and respiratory distress at birth. Unlike other congenital myopathies, the health status constantly improves and patients are minimally affected at adulthood.
  • 配列類似性
    Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily.
    Contains 3 B30.2/SPRY domains.
    Contains 5 MIR domains.
  • ドメイン
    The calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum (SR) and the T-tubule.
  • 翻訳後修飾
    Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2843 may increase channel activity. Repeated very high-level exercise increases phosphorylation at Ser-2843.
    Activated by reversible S-nitrosylation. Repeated very high-level exercise increases S-nitrosylation.
  • 細胞内局在
    Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Arrhythmogenic right ventricular dysplasia 2 antibody
    • ARVC2 antibody
    • ARVD2 antibody
    • Brain ryanodine receptor calcium release channel antibody
    • Brain type ryanodine receptor antibody
    • Cardiac muscle ryanodine receptor calcium release channel antibody
    • Cardiac muscle type ryanodine receptor antibody
    • CCO antibody
    • Central core disease of muscle antibody
    • HBRR antibody
    • hRYR 2 antibody
    • hRYR2 antibody
    • MHS antibody
    • MHS1 antibody
    • Ryanodine receptor 1 (skeletal) antibody
    • Ryanodine receptor 1 antibody
    • Ryanodine receptor 2 (cardiac) antibody
    • Ryanodine receptor 2 antibody
    • Ryanodine receptor 3 antibody
    • Ryanodine receptor type1 antibody
    • RYDR antibody
    • RYR 1 antibody
    • RYR 2 antibody
    • RYR 3 antibody
    • RYR antibody
    • RYR-1 antibody
    • RyR1 antibody
    • RYR1_HUMAN antibody
    • RYR2 antibody
    • RYR3 antibody
    • Sarcoplasmic reticulum calcium release channel antibody
    • Skeletal muscle calcium release channel antibody
    • Skeletal muscle ryanodine receptor antibody
    • Skeletal muscle type ryanodine receptor antibody
    • Skeletal muscle-type ryanodine receptor antibody
    • SKRR antibody
    • Type 1 like ryanodine receptor antibody
    • Type 1 ryanodine receptor antibody
    • VTSIP antibody
    see all

Anti-Ryanodine Receptor antibody [C3-33] 画像

  • ab2827 at 1/100 staining rabbit atrium tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 12 hours. An Alexa-Fluor ® 594 conjugated goat anti-mouse IgG antibody was used as the secondary.

    See Abreview

  • ab2827 staining Ryanodine Receptor in dog skeletal muscle section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone. The primary antibody was diluted 1/100 and incubated with sample for 12 hours at 4°C. A FITC-conjugated goat polyclonal to mouse IgG1, diluted 1/200 was used as secondary.

    See Abreview

Anti-Ryanodine Receptor antibody [C3-33] (ab2827) 使用論文

This product has been referenced in:
  • Popescu I  et al. Elevated local [Ca2+] and CaMKII promote spontaneous Ca2+ release in ankyrin-B-deficient hearts. Cardiovasc Res 111:287-94 (2016). Read more (PubMed: 27131508) »
  • Castillero E  et al. Attenuation of the unfolded protein response and endoplasmic reticulum stress after mechanical unloading in dilated cardiomyopathy. Am J Physiol Heart Circ Physiol 309:H459-70 (2015). WB ; Human . Read more (PubMed: 26055788) »

See all 15 Publications for this product

Product Wall

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Sheep Tissue lysate - whole (cardiac)
Specification
cardiac
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

投稿 Nov 15 2013

Thank you for your reply.

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your pu...

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I am sorry about the issues you were having with ab2877 in western blot. As the antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on rat samples and I do not think ...

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Thank you for contacting Abcam.
I am sorry about the issues you have been having with ab2827 in IF on cultured rat cells. The antibody is covered under our Abpromise for six months and is guaranteed to work in ICC/IF on rat samples.
Based on t...

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Application
Immunohistochemistry (Frozen sections)
Sample
Horse Tissue sections (Skeletal muscles)
Specification
Skeletal muscles
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Verified customer

投稿 Jul 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hESC-derived cardiomyocyte)
Specification
hESC-derived cardiomyocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Verified customer

投稿 Nov 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (skeletal muscle)
Specification
skeletal muscle
Fixative
Acetone
Permeabilization
No
Username

Abcam user community

Verified customer

投稿 May 20 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (atrium)
Specification
atrium
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%
Username

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Verified customer

投稿 Apr 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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