製品の概要

  • 製品名Anti-Ryanodine Receptor antibody [34C]
    Ryanodine Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [34C] to Ryanodine Receptor
  • 特異性Detects Ryanodine Receptor (RyR)-1 and RyR-2 isoforms. In chickens, this antibody detects the alpha, beta and cardiac isoforms. This antibody also detects RyR-3 in mouse cells. In frog, this antibody detects the alpha and beta isoforms. In fish, this antibody detects the alpha isoform. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain results in intense staining of cerebellar Purkinje neurons.
  • アプリケーション適用あり: ICC/IF, IHC-P, IHC-Fr, IP, WB, Inhibition Assaymore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Human, Pig, Xenopus laevis, Non Human Primates
    交差が予測される動物種: Fish, Amphibians
  • 免疫原

    Other Immunogen Type corresponding to Chicken Ryanodine Receptor. Partially purified chicken pectoral muscle ryanodine receptor.

  • ポジティブ・コントロール
    • rat skeletal muscle

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium azide
  • Concentration information loading...
  • 精製度Protein A purified
  • 一次抗体 備考The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.
  • ポリ/モノモノクローナル
  • クローン名34C
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/100.
IHC-Fr 1/100 - 1/1000.
IP Use at an assay dependent concentration.
WB 1/5000. Predicted molecular weight: 565 kDa. As RyR is a large protein, we recommend using a 6-8% gel, wet transfering protein overnight at low voltage, adding 0.1% SDS to transfer buffer and reducing methanol to 10% or less.
Inhibition Assay Use at an assay dependent concentration.

ターゲット情報

  • 機能Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm. Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis.
  • 組織特異性Skeletal muscle and brain (cerebellum and hippocampus).
  • 関連疾患Malignant hyperthermia 1
    Central core disease of muscle
    Multiminicore disease with external ophthalmoplegia
    Myopathy, congenital, with fiber-type disproportion
    Defects in RYR1 may be a cause of Samaritan myopathy, a congenital myopathy with benign course. Patients display severe hypotonia and respiratory distress at birth. Unlike other congenital myopathies, the health status constantly improves and patients are minimally affected at adulthood.
  • 配列類似性Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily.
    Contains 3 B30.2/SPRY domains.
    Contains 5 MIR domains.
  • ドメインThe calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum (SR) and the T-tubule.
  • 翻訳後修飾Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2843 may increase channel activity. Repeated very high-level exercise increases phosphorylation at Ser-2843.
    Activated by reversible S-nitrosylation. Repeated very high-level exercise increases S-nitrosylation.
  • 細胞内局在Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Arrhythmogenic right ventricular dysplasia 2 antibody
    • ARVC2 antibody
    • ARVD2 antibody
    • Brain ryanodine receptor calcium release channel antibody
    • Brain type ryanodine receptor antibody
    • Cardiac muscle ryanodine receptor calcium release channel antibody
    • Cardiac muscle type ryanodine receptor antibody
    • CCO antibody
    • Central core disease of muscle antibody
    • HBRR antibody
    • hRYR 2 antibody
    • hRYR2 antibody
    • MHS antibody
    • MHS1 antibody
    • Ryanodine receptor 1 (skeletal) antibody
    • Ryanodine receptor 1 antibody
    • Ryanodine receptor 2 (cardiac) antibody
    • Ryanodine receptor 2 antibody
    • Ryanodine receptor 3 antibody
    • Ryanodine receptor type1 antibody
    • RYDR antibody
    • RYR 1 antibody
    • RYR 2 antibody
    • RYR 3 antibody
    • RYR antibody
    • RYR-1 antibody
    • RyR1 antibody
    • RYR1_HUMAN antibody
    • RYR2 antibody
    • RYR3 antibody
    • Sarcoplasmic reticulum calcium release channel antibody
    • Skeletal muscle calcium release channel antibody
    • Skeletal muscle ryanodine receptor antibody
    • Skeletal muscle type ryanodine receptor antibody
    • Skeletal muscle-type ryanodine receptor antibody
    • SKRR antibody
    • Type 1 like ryanodine receptor antibody
    • Type 1 ryanodine receptor antibody
    • VTSIP antibody
    see all

Anti-Ryanodine Receptor antibody [34C] 画像

  • ICC/IF image of ab2868 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2868, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab2868 staining Ryanodine Receptor (red) in Mouse Skeletal muscle cells at day 10 of agrin-treated differentation by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 10% serum for 45 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) for 2 hours at 20°C. An Alexa Fluor® 647-conjugated Donkey anti-goat IgG polyclonal (1/500) was used as the secondary antibody. Nucleus stained blue

    See Abreview

  • Immunohistochemistry was performed on biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Ryanodine Receptor ab2868 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • ab2868 at a 1/200 dilution staining Ryanodine Receptor in mouse liver tissue sections by Immunohistochemistry (frozen sections) incubated for 9 hours at +4°C. Fixed in formaldehyde, permeabilized using 0.2% Triton X-100. Blocked using 2% BSA for 30 minutes at 20°C. Secondary used at a 1/200 dilution polyclonal Goat anti-mouse IgG conjugated to Alexa Fluor 555.

    See Abreview



  • Predicted band size : 565 kDa

    Image from Blaich A et al., J Biol Chem 2012 Jun 29;287(27):22616-25. Epub 2012 May 15.; Fig 5.; doi: 10.1074/jbc.M111.335927; June 29, 2012 The Journal of Biological Chemistry, 287, 23216-23226.

    Representative Western blots for Ser(P)2814 and Ryanodine Receptor in control and Cav1.2I/E mutant mouse hearts. Ryanodine Receptor (bottom panel) was detected with ab2868.
  • ab2868 staining Ryanodine Receptor in Rat cardiomyocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1) and blocked with 3% BSA for 1 hour at 18°C. Samples were incubated with primary antibody (1/300 in PBS + 3% BSA) for 16 hours at 4°C. An Alexa Fluor® 546-conjugated Goat anti-mouse IgG (H+L) polyclonal (1/300) was used as the secondary antibody.

    See Abreview

Anti-Ryanodine Receptor antibody [34C] (ab2868) 使用論文

This product has been referenced in:
  • Yang W  et al. Calcium concentration response to uterine ischemia: a comparison of uterine fibroid cells and adjacent normal myometrial cells. Eur J Obstet Gynecol Reprod Biol 174:123-7 (2014). Human . Read more (PubMed: 24405728) »
  • Wright MF  et al. Mechanisms of intracellular calcium homeostasis in developing and mature bovine corpora lutea. Biol Reprod 90:55 (2014). WB, IHC-P ; Cow . Read more (PubMed: 24501170) »

See all 22 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (cardiomyocyte)
Permeabilization No
Specification cardiomyocyte
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
Fixative 50%acetone + 50%methanol
Username

Miss. Can Zhou

Verified customer

投稿 Apr 04 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Skeletal muscle)
Permeabilization Yes - 0.25% Triton/PBS
Specification Skeletal muscle
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Ms. Jenny Sharpe

Verified customer

投稿 Oct 09 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Cell (myocyte)
Specification myocyte
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Jun 11 2013

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Pig Tissue lysate - whole (muscle)
Specification muscle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 07 2013

I am sorry to hear you have been experiencing problems with our antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints.

I would like to investigate this particul...

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Thank you for email.

This antibody is very well charaterized. We know this antibody detects RyR-3 in mouse cells and by Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts.

The Ry...

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Thank you for your reply.


We have a number of phospho specific antibodies in our catalog, but ab59225 is the only phospho specific antibody we have to Ryanodine Receptors.


In order to determine specifically which residue...

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Vielen Dank für Ihren Anruf. Das Labor hat mir mitgeteilt, dass für den Western blot ein 6- oder 8%iges Acrylamidgel empfohlen wird und der Transfer 'wet' (d.h. 'not fast or semi-dry') erfolgen sollte. Dies kann bei 90 Volt für 2 Stunden oder niedriger...

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Thank you for your inquiry. 1. Thank you for making is aware of this typo. This has been corrected. 2. We only are aware of a publication for chicken where this clone of antibody was used: J Cell Biol. 1991 Jun;113(5):1145-57. "Ryanodine and ...

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Thank you for your enquiry regarding ab2868 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. This antibody is cited in many publications and Abrev...

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1-10 of 22 Abreviews or Q&A

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