製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RWDD4A
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 139 - 188 (SKKKDKKEQL SKAQKRKLAD KTDHKGELPR GWNWVDVVKH LSKTGSKDDE) of Human RWDD4A (NP_689895)

  • ポジティブ・コントロール
    • SH-SYSY cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab90065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-RWDD4A antibody 画像

  • Anti-RWDD4A antibody (ab90065) at 1 µg/ml + SH-SYSY cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 21 kDa

プロトコール

Anti-RWDD4A antibody (ab90065) 使用論文

ab90065 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount
120 µg
Specification
Human osteosarcoma U2OS cells
Gel Running Conditions
Reduced Denaturing (4-18% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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