Rabbit polyclonal to RUNX3
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Mouse RUNX3.
(Peptide available as
This antibody gave a positive signal in Jurkat and RAW 264.7 Whole Cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
Contains 1 Runt domain.
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm.
Information by UniProt
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Western blot - RUNX3 antibody (ab83500)
All lanes : Anti-RUNX3 antibody (ab83500) at 1 µg/ml Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 44 kDa Observed band size : 44 kDa Additional bands at : 53 kDa. We are unsure as to the identity of these extra bands. Exposure time : 16 minutes
Immunocytochemistry/ Immunofluorescence - RUNX3 antibody (ab83500)
ICC/IF image of ab83500 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83500, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml.
has not yet been referenced specifically in any publications.
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