製品の概要

  • 製品名Anti-RUNX3 antibody
    RUNX3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RUNX3
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Guinea pig, Cat, Dog
  • 免疫原

    A region within synthetic peptide: GAPQSQSGPF QANPAPYHLF YGASSGSYQF SMAAAGGGER SPTRMLTSCP, corresponding to C terminal amino acids 326-375 of Mouse RUNX3

  • ポジティブ・コントロール
    • NIH3T3 cell lysates

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab49117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/12500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration. PubMed: 21205319

ターゲット情報

  • 機能CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
  • 配列類似性Contains 1 Runt domain.
  • ドメインA proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
  • 翻訳後修飾Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
  • 細胞内局在Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acute myeloid leukemia 2 protein antibody
    • Acute myeloid leukemia gene 2 antibody
    • AML 2 antibody
    • AML2 antibody
    • CBF alpha 3 antibody
    • CBF-alpha-3 antibody
    • CBFA 3 antibody
    • CBFA3 antibody
    • Core binding factor alpha 3 subunit antibody
    • core binding factor antibody
    • Core binding factor runt domain alpha subunit 3 antibody
    • Core binding factor subunit alpha 3 antibody
    • core-binding factor antibody
    • Core-binding factor subunit alpha-3 antibody
    • Oncogene AML 2 antibody
    • Oncogene AML-2 antibody
    • PEA2 alpha C antibody
    • PEA2-alpha C antibody
    • PEBP2 alpha C antibody
    • PEBP2-alpha C antibody
    • Pebp2a3 antibody
    • PEBP2aC antibody
    • Polyomavirus enhancer binding protein 2 alpha C subunit antibody
    • Polyomavirus enhancer-binding protein 2 alpha C subunit antibody
    • runt domain alpha subunit 3 antibody
    • runt related transcription factor 3 antibody
    • Runt-related transcription factor 3 antibody
    • RUNX 3 antibody
    • Runx3 antibody
    • RUNX3_HUMAN antibody
    • SL3 3 enhancer factor 1 alpha C subunit antibody
    • SL3-3 enhancer factor 1 alpha C subunit antibody
    • SL3/AKV core binding factor alpha C subunit antibody
    • SL3/AKV core-binding factor alpha C subunit antibody
    • Transcription factor AML2 antibody
    see all

Anti-RUNX3 antibody 画像

  • Anti-RUNX3 antibody (ab49117) at 1.25 µg/ml + NIH3T3 cell lysate at 10 µg

    Predicted band size : 44 kDa
    Observed band size : 44 kDa

Anti-RUNX3 antibody (ab49117) 使用論文

This product has been referenced in:
  • Wang Z  et al. Epigenetic reprogramming of human lung cancer cells with the extract of bovine parthenogenetic oocytes. J Cell Mol Med 18:1807-15 (2014). WB . Read more (PubMed: 24889513) »
  • Nakanishi Y  et al. Loss of runt-related transcription factor 3 expression leads hepatocellular carcinoma cells to escape apoptosis. BMC Cancer 11:3 (2011). WB, IHC-P ; Human . Read more (PubMed: 21205319) »

See all 3 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 120 µg
Gel Running Conditions Reduced Denaturing (12,5)
Sample Zebrafish Tissue lysate - whole (whole embryo lysate)
Specification whole embryo lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 25 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human osteosarcoma)
Specification Human osteosarcoma
Fixative Paraformaldehyde
Permeabilization Yes - PBS TRITON 0.25%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 May 11 2012

Thank you for your reply.


Below please find the recipe for 1mM EDTA, adjusted to pH 8.0:


EDTA 0.37 g
Distilled water 1000 ml
Store at room temperature for 3 months.


Tris would not be used to pH ...

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Thank you for your inquiry.

For ab49117, we would guarantee this antibody to work in IHC-P in mouse, however we have not tested this in house. We know that IHC-P has been tested with human samples in this reference:

Nakanishi Y et a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"