リコンビナント
RabMAb

Anti-RUNX1 / AML1 + RUNX3 + RUNX2 抗体 [EPR3099] (ab92336)

製品の概要

  • 製品名
    Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099]
    RUNX1 / AML1+RUNX3+RUNX2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3099] to RUNX1 / AML1 + RUNX3 + RUNX2
  • アプリケーション
    適用あり: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human RUNX1/ AML1 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab177141)

  • ポジティブ・コントロール
    • WB: MOLT4 cell lysate, fetal thymus tissue lysate. IHC: Human tonsil tissue.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Predicted molecular weight: 49 kDa.Can be blocked with RUNX1 / AML1 peptide (ab177141).
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody will give a stronger signal.

Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.

ターゲット情報

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  • Developed using the ECL technique

    Predicted band size : 49 kDa


    Exposure time : 1 minute

    RUNX2 recombinant protein full length, with N-terminal HIS tag, expressed in E.Coli.

    RUNX3 overexpression and empty vector control lysates created in HEK293T cells.  The protein contains a C-terminal DDK tag.

  • All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1/10000 dilution

    Lane 1 : MOLT4 cell lysate
    Lane 2 : fetal thymus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 49 kDa
  • Immunohistochemistry staining of RUNX1 / AML1 in formalin-fixed, paraffin-embedded Human tonsil tissue using 1/100 ab92336.
  • Flow cytometric analysis of permeabilized Molt-4 cells using anti-RUNX1 ab92336 (red) or a rabbit IgG (negative) (green).
  • ab92336 staining RUNX1 / AML1 in human glioblastoma cell line by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab92336 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

  • ab92336 staining RUNX1 / AML1 in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab92336 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.

    See Abreview

参考文献

This product has been referenced in:
  • Batsivari A  et al. Understanding Hematopoietic Stem Cell Development through Functional Correlation of Their Proliferative Status with the Intra-aortic Cluster Architecture. Stem Cell Reports 8:1549-1562 (2017). IF ; Mouse . Read more (PubMed: 28479304) »
  • Lilly AJ  et al. SOX7 expression is critically required in FLK1-expressing cells for vasculogenesis and angiogenesis during mouse embryonic development. Mech Dev 146:31-41 (2017). IHC-P ; Mouse . Read more (PubMed: 28577909) »

See all 19 Publications for this product

レビューと Q&A

Merci de nous avoir contactés.

J'ai donc comme convenu vérifié les séquences immunogènes et les alignements de séquences (voir fichiers joints)pour les deux monoclonaux de lapin anti-RUNX1 / AML1:
...

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Merci pour votre appel.

J'ai fait l'alignement de l-ab92336 avec la séquence de poulet, mais c'est pas super. Veuillez voir vous-même: http://www.ebi.ac.uk/Tools/services/web_clustalo/toolresult.ebi?tool=clustalo&jobId=clusta...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line U87MG)
Specification
human glioblastoma cell line U87MG
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% Triton X 100 in PBS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (glioblastoma cell line from rat)
Specification
glioblastoma cell line from rat
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% Triton X 100 in PBS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (glioblastoma cell line C6)
Specification
glioblastoma cell line C6
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line)
Specification
human glioblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton X100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 24 2012

Thank you for bringing this to our attention.  I have forwarded your data to our lab for their input.  We may end up submitting a recall for this product, but I would like for them to review your results.  I have refunded your purchase. I am sorry...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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