製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RUNDC1
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 354-461 of Human RUNDC1.

  • ポジティブ・コントロール
    • Human lymph node tissue; RT 4 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

ターゲット情報

画像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121773 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121773 at 1/20 dilution staining RUNDC1 in Paraffin-embedded Human lymph node tissue by Immunohistochemistry.
  • All lanes : Anti-RUNDC1 antibody (ab121773) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique

参考文献

ab121773 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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