製品の概要

  • 製品名Anti-RUFY1 antibody
    RUFY1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to RUFY1
  • 特異性ab109804 is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical proteins: NP_001035542.1 and NP_001035541.1.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    C-QKNEAITSFEGKT

    , corresponding to internal sequence amino acids 479-491 of Human RUFY1 (NP_079434.3; NP_001035541.1).

  • ポジティブ・コントロール
    • HeLa lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).

ターゲット情報

  • 機能Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
  • 組織特異性Broadly expressed, with highest levels in lung, testis, kidney and brain.
  • 配列類似性Contains 1 FYVE-type zinc finger.
    Contains 1 RUN domain.
  • ドメインThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • 翻訳後修飾Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3000002E04Rik antibody
    • FLJ22251 antibody
    • FYVE finger protein EIP1 antibody
    • FYVE-finger protein EIP1 antibody
    • La binding protein 1 antibody
    • La-binding protein 1 antibody
    • OTTHUMP00000161521 antibody
    • Rab4 interacting protein antibody
    • Rab4-interacting protein antibody
    • RABIP4 antibody
    • RUFY1 antibody
    • RUFY1_HUMAN antibody
    • RUN and FYVE domain containing 1 antibody
    • RUN and FYVE domain-containing protein 1 antibody
    • RUN and FYVE domains containing protein 1 antibody
    • ZFYVE12 antibody
    • Zinc finger FYVE domain containing protein 12 antibody
    • Zinc finger FYVE domain-containing protein 12 antibody
    see all

Anti-RUFY1 antibody 画像

  • Anti-RUFY1 antibody (ab109804) at 0.05 µg/ml + HeLa lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 80 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

Anti-RUFY1 antibody (ab109804) 使用論文

ab109804 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab109804.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"