製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to RUBISCO
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Spinacia oleracea
    交差が予測される動物種: Plants
  • 免疫原

    Full length protein; RUBISCO small subunit (Spinach). NCBI Acc : AAC29137.1, Swiss-Prot Q9TIM3.

  • ポジティブ・コントロール
    • Purified spinach RUBISCO.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 15, 45 kDa (predicted molecular weight: 55 kDa).

ターゲット情報

  • 機能
    RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
  • 配列類似性
    Belongs to the RuBisCO large chain family. Type I subfamily.
  • 翻訳後修飾
    The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.
  • 細胞内局在
    Plastid > chloroplast.
  • Information by UniProt
  • 参照データベース
    • 別名
      • 3 phospho D glycerate carboxylase antibody
      • rbcL antibody
      • RBL_SPIOL antibody
      • Ribulose 1,5 bisphosphate carboxylase/oxygenase small subunit N methyltransferase I antibody
      • Ribulose bisphosphate carboxylase large chain antibody
      • RuBisCO large subunit antibody
      see all

    画像

    • Anti-RUBISCO antibody (ab62391) at 1/2000 dilution + Purified spinach RUBISCO protein at 1 µg

      Secondary
      Goat anti-IgY-HRP at 1/1000 dilution

      Predicted band size : 55 kDa
      Observed band size : 15,45 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    This product has been referenced in:
    • Cai Z  et al. Development of an activity-directed selection system enabled significant improvement of the carboxylation efficiency of Rubisco. Protein Cell 5:552-62 (2014). WB . Read more (PubMed: 24870149) »

    See 1 Publication for this product

    レビューと Q&A

    Thank you for contacting us with your questions. We have only tested these antibodies under reducing conditions in WB. They may be suitable for use under non-reducing conditions as well, but this has not been validated in-house. I hope this information...

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    Thank you for contacting us.  We have only tested these Peroxiredoxin antibodies under reducing conditions in WB.  They may be suitable for use under non-reducing conditions as well, but this has not been validated in house.  The unconjugated secondary...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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