製品の概要

  • 製品名Anti-RTN3 antibody
    RTN3 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to RTN3
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    RTN3 (NP_006045.1, 1 a.a. ~ 236 a.a) full length human protein.

  • ポジティブ・コントロール
    • RTN3 transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa for isoform 3).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis.
    • 組織特異性Isoform 3 is widely expressed, with highest levels in brain, where it is enriched in neuronal cell bodies from gray matter (at protein level). Three times more abundant in macula than in peripheral retina. Isoform 1 is expressed at high levels in brain and at low levels in skeletal muscle. Isoform 2 is only found in melanoma.
    • 配列類似性Contains 1 reticulon domain.
    • 細胞内局在Endoplasmic reticulum membrane. Golgi apparatus membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ASYIP antibody
      • Neuroendocrine specific protein like 2 antibody
      • Neuroendocrine-specific protein-like 2 antibody
      • Neuroendocrine-specific protein-like II antibody
      • NSP like protein II antibody
      • NSP-like protein 2 antibody
      • NSP-like protein II antibody
      • NSPL2 antibody
      • NSPLII antibody
      • Reticulon 3 antibody
      • Reticulon-3 antibody
      • Reticulon3 antibody
      • Rtn3 antibody
      • RTN3_HUMAN antibody
      see all

    Anti-RTN3 antibody 画像

    • All lanes : Anti-RTN3 antibody (ab68328) at 1/500 dilution

      Lane 1 : RTN3 transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 26 kDa
      Observed band size : 22 kDa (why is the actual band size different from the predicted?)

    Anti-RTN3 antibody (ab68328) 使用論文

    ab68328 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Hela)
    Permeabilization Yes - 0.5% Triton X-100
    Specification Hela
    Blocking step Fish Skin Gelatin (FSG) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
    Fixative Paraformaldehyde
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    投稿 Nov 05 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"