製品の概要

  • 製品名Anti-RT1-Aw2 antibody [MRC OX-18]
    RT1-Aw2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MRC OX-18] to RT1-Aw2
  • 特異性ab119782 recognizes a monomorphic determinant of rat Class I MHC antigen (RT1.A) and thus reacts with all rat strains tested including AO(RT1µ), DA (RT1a), LEW (RT11) and PVG (RT1c).
  • アプリケーション適用あり: Flow Cyt, WBmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Rat spleen membrane glycoproteins depleted of Ia-A antigens.

  • ポジティブ・コントロール
    • Thymus (38.1% cells stained); Spleen (97.0% cells stained); Lymph Node (99.9% cells stained).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.02% Sodium azide
    Constituents: BSA, PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)Purified from ascitic fluid
  • ポリ/モノモノクローナル
  • クローン名MRC OX-18
  • ミエローマx63-Ag8.653
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab119782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.2-0.5µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration.

ターゲット情報

  • 機能Involved in the presentation of foreign antigens to the immune system.
  • 配列類似性Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Class I histocompatibility antigen antibody
    • HA11_RAT antibody
    • MHCRTA antibody
    • Non-RT1.A alpha-1 chain antibody
    • RATMHCRTA antibody
    • RT1 A antibody
    • RT1-Aw2 antibody
    • RT1.EC2 antibody
    • RT1A antibody
    • RTS antibody
    see all

Anti-RT1-Aw2 antibody [MRC OX-18] 画像

  • Rat Strain: Wistar
    Cell Concentration: 1x106 cells
    Antibody Concentration Used: 0.2 µg/106 cells
    Isotypic Control: Mouse IgG
    Cell Source: Spleen
    Percentage of cells stained: 97.0% .

Anti-RT1-Aw2 antibody [MRC OX-18] (ab119782) 使用論文

This product has been referenced in:
  • Fukumoto T  et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol 12:237-43 (1982). Read more (PubMed: 6178598) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Rat Tissue lysate - whole (Hypothalamus)
Specification Hypothalamus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (hippocampus)
Loading amount 20 µg
Specification hippocampus
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Jana Aradska

Verified customer

投稿 Aug 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"