Anti-RSPO3 抗体 (ab109808)

製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to RSPO3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    QHPSAKGNLCPPT

    , corresponding to internal sequence amino acids 181-193 of Human RSPO3 (NP_116173.2). RSPO3(Peptide available as ab135941.)

  • ポジティブ・コントロール
    • Human skeletal muscle lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).Can be blocked with Human RSPO3 peptide (ab135941).

ターゲット情報

  • 機能
    Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway.
  • 組織特異性
    Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node.
  • 配列類似性
    Belongs to the R-spondin family.
    Contains 2 FU (furin-like) repeats.
    Contains 1 TSP type-1 domain.
  • ドメイン
    The FU repeats are required for activation and stabilization of beta-catenin.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CRISTIN1 antibody
    • hPWTSR antibody
    • hRspo3 antibody
    • Protein with TSP type-1 repeat antibody
    • PWTSR antibody
    • R-spondin 3 homolog antibody
    • R-spondin-3 [Precursor] antibody
    • R-spondin-3 antibody
    • Roof plate specific spondin-3 antibody
    • Roof plate-specific spondin-3 antibody
    • RSPO3 antibody
    • RSPO3_HUMAN antibody
    • Thrombospondin type I domain containing 2 antibody
    • Thrombospondin type-1 domain-containing protein 2 antibody
    • THSD2 antibody
    see all

画像

  • Anti-RSPO3 antibody (ab109808) at 0.3 µg/ml + Human skeletal muscle lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 31 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

プロトコール

参考文献

ab109808 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse embryo section)
Specification
mouse embryo section
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
Blocking step
NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 15 2013

I would like to inform you that we have been able to place the RSPO3 peptide used to create ab190808 on to our catalog. The product number for this is ab135945. The datasheet for this product may be found at the following address: http://www.abcam.c...

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Thank you for contacting us.

I have contacted the lab to get this peptide added to our catalog. These may take a few days but I will let you know when it has been added.

I hope this information is helpful to you. Please do not...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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