製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RSPO2
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RSPO2.

    (Peptide available as ab98902.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: LOVO; SW480; Caco 2; HCT 116

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab73761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).

ターゲット情報

  • 関連性
    RSPO2 is part of the R-spondin family of proteins that consist of secreted cysteine-rich proteins that can activate beta-catenin signaling via the Frizzled/LRP5/6 receptor complex. R-spondin 2 is required for normal laryngeal-tracheal, lung and limb morphogenesis. RSPO2 expression in required in the apical ectodermal ridge for normal hindlimb development.
  • 細胞内局在
    Secreted
  • 参照データベース
  • 別名
    • CRISTIN2 antibody
    • hRspo2 antibody
    • R spondin 2 antibody
    • R spondin 2 homolog antibody
    • R-spondin 2 homolog (Xenopus laevis) antibody
    • R-spondin family, member 2 antibody
    • Roof plate-specific spondin-2 antibody
    see all

Anti-RSPO2 antibody 画像

  • All lanes : Anti-RSPO2 antibody (ab73761) at 1 µg/ml

    Lane 1 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 4 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 106 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ab73761 staining RSPO2 in human meniscus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed using Protease24. Samples were then blocked using 1% BSA for 15 minutes at 21°C, then incubated with ab73761 at a 1/100 dilution for 16 hours at 21°C. The secondary used was an undiluted biotin conjugated goat anti-rabbit polyclonal.

    See Abreview

Anti-RSPO2 antibody (ab73761) 使用論文

This product has been referenced in:
  • McCullough KM  et al. Molecular characterization of Thy1 expressing fear-inhibiting neurons within the basolateral amygdala. Nat Commun 7:13149 (2016). IHC-FrFl ; Mouse . Read more (PubMed: 27767183) »
  • Yan H  et al. Rspo2 suppresses CD36-mediated apoptosis in oxidized low density lipoprotein-induced macrophages. Mol Med Rep 14:2945-52 (2016). WB ; Human . Read more (PubMed: 27571704) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (meniscus)
Loading amount
200 µg
Specification
meniscus
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Aug 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (meniscus)
Specification
meniscus
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Protease24
Permeabilization
No
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Jun 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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