Anti-RSPH4A 抗体 (ab122157)
Key features and details
- Rabbit polyclonal to RSPH4A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RSPH4A antibody
RSPH4A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RSPH4A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
antigen sequence, corresponding to amino acids 22-94 (RPWEGKTAAS PQYSEPESSE PLEAKQGPET GRQSRSSRPW SPQSRAKTPL GGPAGPETSS PAPVSPREPS SSP) of Human RSPH4A.
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ポジティブ・コントロール
- Human bronchus tissue; RT-4 and U-251 MG cell lysates; Human liver and tonsil lysates; A-431 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122157の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/250 - 1/500.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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特記事項 |
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WB
1/250 - 1/500. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
ターゲット情報
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機能
Probable component of the axonemal radial spoke head. Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk which is attached to a subfiber of the outer doublet microtubule, and a bulbous head which is attached to the stalk and appears to interact with the projections from the central pair of microtubules. -
関連疾患
Defects in RSPH4A are the cause of primary ciliary dyskinesia type 11 (CILD11) [MIM:612649]. CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. -
配列類似性
Belongs to the flagellar radial spoke RSP4/6 family. -
細胞内局在
Cytoplasm > cytoskeleton > cilium axoneme. Radial spoke. - Information by UniProt
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参照データベース
- Entrez Gene: 345895 Human
- Omim: 612647 Human
- SwissProt: Q5TD94 Human
- Unigene: 160380 Human
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別名
- A230081C05 antibody
- CILD11 antibody
- dJ412I7.1 antibody
see all
画像
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ab122157, at 4 µg/ml, staining RSPH4A (green) in PFA-fixed, Triton-permeabilized Human A-431 cells by Immunofluorescence.
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All lanes : Anti-RSPH4A antibody (ab122157) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique. -
ab122157, at 1/75, staining RSPH4A in paraffin embedded Human bronchus tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab122157 は論文での使用が確認できていません。