製品の概要

  • 製品名Anti-RSPH3 antibody
  • 製品の詳細
    Rabbit polyclonal to RSPH3
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence corresponding to amino acids 268-354 (IIEVDMECQT DAFLDRPPTP LFIPAKTGKD VATQILEGEL FDFDLEVKPV LEVLVGKTIE QSLLEVMEEE ELANLRASQR EYEELRN) of Human RSPH3, isoform 1.

  • ポジティブ・コントロール
    • Human testis tissue; RT-4 and U-251 MG cell lysates; Human Plasma, Liver and Tonsil lysates

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

ターゲット情報

  • 配列類似性Belongs to the flagellar radial spoke RSP3 family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ111C20.1 antibody
    • Radial spoke 3 homolog (Chlamydomonas) antibody
    • Radial spoke head 3 homolog antibody
    • Radial spoke head protein 3 homolog antibody
    • Radial spoke head-like protein 2 antibody
    • Radial spoke protein 3 antibody
    • Radial spokehead like 2 antibody
    • RSHL2 antibody
    • RSP3 antibody
    • RSPH3 antibody
    • RSPH3_HUMAN antibody
    see all

Anti-RSPH3 antibody 画像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122535 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122535, at 1/250 dilution, staining RSPH3 in paraffin-embedded Human testis tissue by Immunohistochemistry, showing moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
  • All lanes : Anti-RSPH3 antibody (ab122535) at 1/250 dilution

    Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human Plasma lysate
    Lane 5 : Human Liver lysate
    Lane 6 : Human Tonsil lysate

Anti-RSPH3 antibody (ab122535) 使用論文

ab122535 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122535.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"