Anti-RSK4 (phospho S232) 抗体 [EP1524Y] (ab81290)
Key features and details
- Rabbit monoclonal [EP1524Y] to RSK4 (phospho S232)
- Suitable for: WB, IHC-P, ELISA, Dot blot
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-RSK4 (phospho S232) antibody [EP1524Y]
RSK4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1524Y] to RSK4 (phospho S232) -
由来種
Rabbit -
特異性
Due to high sequence homology, this antibody might cross-react with RSK1(Q15418) and RSK3(P51812).
This antibody cross-reactivates with PSK3 (phospho S218). The immunogen used for this product shares 100% homology with RSK1 and RSK2, Cross-reactivity with these two proteins has not been confirmed experimentally.
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アプリケーション
適用あり: WB, IHC-P, ELISA, Dot blotmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Hela cell lysate IHC-P: human muscle tissue.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1524Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81290の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ELISA |
Use at an assay dependent concentration.
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Dot blot |
1/1000.
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特記事項 |
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WB
1/5000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ELISA
Use at an assay dependent concentration. |
Dot blot
1/1000. |
ターゲット情報
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機能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻訳後修飾
Autophosphorylated on Ser-389, as part of the activation process. - Information by UniProt
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参照データベース
- Entrez Gene: 27330 Human
- Entrez Gene: 67071 Mouse
- Omim: 300303 Human
- SwissProt: Q9UK32 Human
- SwissProt: Q7TPS0 Mouse
- Unigene: 234478 Human
- Unigene: 37617 Mouse
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別名
- 90 kDa ribosomal protein S6 kinase 6 antibody
- KS6A6_HUMAN antibody
- p90 RSK 6 antibody
see all
画像
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All lanes : Anti-RSK4 (phospho S232) antibody [EP1524Y] (ab81290) at 1/10000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate untreated
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa -
ab81290, at 1/50, staining human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Direct ELISA antigen dose-response curve using ab81290.
Primary antibody: ab81290 (0-1000 ng/ml).
Antigens:
RSK3 (phospho S218) phopspho peptide
RSK3 non-phopspho peptide
RSK4 (phospho S232) phopspho peptide
RSK4 non-phopspho peptide
BSA-branched PEG 40k (0-100 ng/ml).
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.
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Dot blot analysis of RSK4 (pS232) phospho peptide (Lane 1), RSK4 non-phospho peptide (Lane 2), RSK3 (pS218) phospho peptide (Lane 3) and RSK3 non-phospho peptide (Lane 4) labeling RSK4 (phospho S232) with ab81290 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab81290 は論文での使用が確認できていません。