製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RSK4
  • 特異性
    Detects endogenous levels of total RSK4 protein
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    synthesized peptide derived from internal of human S6K-a6

  • ポジティブ・コントロール
    • Human brain tissue K562 cell extract HT29 cell extract

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab69929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • 翻訳後修飾
    Autophosphorylated on Ser-389, as part of the activation process.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 90 kDa ribosomal protein S6 kinase 6 antibody
    • KS6A6_HUMAN antibody
    • p90 RSK 6 antibody
    • p90-RSK 6 antibody
    • p90RSK6 antibody
    • pp90RSK4 antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 6 antibody
    • Ribosomal protein S6 kinase alpha 6 antibody
    • Ribosomal protein S6 kinase alpha-6 antibody
    • Ribosomal S6 kinase 4 antibody
    • rps6ka6 antibody
    • RSK 4 antibody
    • RSK-4 antibody
    • S6K-alpha-6 antibody
    see all

画像

  • Immunohistochemical analysis of paraffin embedded human brain tissue treated with ab69929 at 1/50-1/100 dilution. Right panel was treated with immunising peptide.
  • All lanes : Anti-RSK4 antibody (ab69929) at 1/500 dilution

    Lane 1 : K562 cell extract
    Lane 2 : HT29 cell extract
    Lane 3 : HT29 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Additional bands at : 30 kDa,45 kDa. We are unsure as to the identity of these extra bands.

参考文献

ab69929 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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