製品の概要

  • 製品名
    Anti-RSK3 antibody [3C1]
    RSK3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3C1] to RSK3
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 415-733 of Human RSK3, purified from E.coli with His tag.

  • ポジティブ・コントロール
    • WB: HeLa, A431, PC12 and L929 cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 83 kDa).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.
  • 組織特異性
    Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • 翻訳後修飾
    Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1.
    N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
  • 細胞内局在
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 90 kDa ribosomal protein S6 kinase 2 antibody
    • HU 2 antibody
    • KS6A2_HUMAN antibody
    • MAP kinase activated protein kinase 1c antibody
    • MAP kinase-activated protein kinase 1c antibody
    • MAPK-activated protein kinase 1c antibody
    • MAPKAP kinase 1c antibody
    • MAPKAPK-1c antibody
    • MAPKAPK1C antibody
    • Mitogen.activated protein kinase-activated protein kinase 1C antibody
    • p90 RSK3 antibody
    • p90-RSK 2 antibody
    • p90RSK2 antibody
    • pp90RSK3 antibody
    • Ribosomal protein S6 kinase alpha-2 antibody
    • ribosomal protein S6 kinase, 90kDa, polypeptide 2 antibody
    • Ribosomal S6 kinase 3 antibody
    • RPS6KA2 antibody
    • RSK 3 antibody
    • RSK antibody
    • RSK-3 antibody
    • S6K alpha antibody
    • S6K alpha 2 antibody
    • S6K-alpha-2 antibody
    see all

画像

  • All lanes : Anti-RSK3 antibody [3C1] (ab70901) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : L929 cell lysate


    Predicted band size : 83 kDa
  • All lanes : Anti-RSK3 antibody [3C1] (ab70901) at 1/1000 dilution

    Lane 1 : RSK1 recombinant protein
    Lane 2 : RSK2 recombinant protein
    Lane 3 : RSK3 recombinant protein


    Predicted band size : 83 kDa

    Incubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.

プロトコール

参考文献

ab70901 has not yet been referenced specifically in any publications.

レビューと Q&A

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