製品の概要

  • 製品名Anti-RSK1 p90 (phospho T573) antibody [EP2133Y]
    RSK1 p90 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2133Y] to RSK1 p90 (phospho T573)
  • アプリケーション適用あり: ICC/IF, WBmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RSK1 p90.

  • ポジティブ・コントロール
    • K562 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • 翻訳後修飾Autophosphorylated on Ser-380, as part of the activation process.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 90 kDa ribosomal protein S6 kinase 1 antibody
      • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
      • dJ590P13.1 antibody
      • EC 2.7.11.1 antibody
      • HU 1 antibody
      • HU1 antibody
      • KS6A1_HUMAN antibody
      • MAP kinase activated protein kinase 1a antibody
      • MAP kinase-activated protein kinase 1a antibody
      • MAPK-activated protein kinase 1a antibody
      • MAPKAP kinase 1a antibody
      • MAPKAPK-1a antibody
      • MAPKAPK1A antibody
      • MGC79981 antibody
      • Mitogen-activated protein kinase-activated protein kinase 1A antibody
      • OTTHUMP00000004113 antibody
      • p90 RSK1 antibody
      • p90-RSK 1 antibody
      • p90rsk antibody
      • p90RSK1 antibody
      • p90S6K antibody
      • pp90RSK1 antibody
      • Ribosomal protein S6 kinase 90kD 1 antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
      • Ribosomal protein S6 kinase alpha 1 antibody
      • Ribosomal protein S6 kinase alpha-1 antibody
      • Ribosomal protein S6 kinase polypeptide 1 antibody
      • Ribosomal S6 kinase 1 antibody
      • RPS6K1 alpha antibody
      • rps6ka antibody
      • Rps6ka1 antibody
      • RSK 1 antibody
      • RSK 1 p90 antibody
      • RSK antibody
      • RSK-1 antibody
      • RSK1 antibody
      • S6K alpha 1 antibody
      • S6K-alpha-1 antibody
      see all

    Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] 画像

    • All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/2000 dilution (purified)

      Lane 1 : Untreated Ramos whole cell lysate
      Lane 2 : Ramos treated with Phorbol-12-myristate-13-acetate whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 83 kDa
      Observed band size : 83 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescence staining of K562 cells with purified ab62324 at a working dilution of 1/500, counter-stained with DAPI. The cells were treated with 500 nM TPA for 15 minutes. Both the treated and untreated cells are shown. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the right hand panels. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom panels - for negative control 1, purified ab62324 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/500 dilution (Unpurified)

      Lane 1 : K562 cell lysate; untreated
      Lane 2 : K562 cell lysate; treated with TPA

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP conjugated, at 1/2000 dilution

      Predicted band size : 83 kDa
      Observed band size : 83 kDa
      Beta Tubulin has been included as a loading control.

    Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) 使用論文

    This product has been referenced in:
    • Chaikuad A  et al. A unique inhibitor binding site in ERK1/2 is associated with slow binding kinetics. Nat Chem Biol 10:853-60 (2014). Read more (PubMed: 25195011) »
    • Talbot AL  et al. Aconitase regulation of erythropoiesis correlates with a novel licensing function in erythropoietin-induced ERK signaling. PLoS One 6:e23850 (2011). WB . Read more (PubMed: 21887333) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"