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RabMAb

Anti-RSK1 p90 (phospho T359 + S363) 抗体 [E238] (ab32413)

製品の概要

  • 製品名
    Anti-RSK1 p90 (phospho T359 + S363) antibody [E238]
    RSK1 p90 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E238] to RSK1 p90 (phospho T359 + S363)
  • 特異性
    This antibody is specific for human RSK1 p90 phoshorylated on Threonine 359 and Serine 363. 80% identities with RSK3 p90 (pT365+pS369), 75% with RSK2 p90 (pT356+pS360) and 74% identities with RSK6 p90 (pT368+pS372)
  • アプリケーション
    適用あり: WB, IHC-P, ICC, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RSK1 p90 aa 350-450.

  • ポジティブ・コントロール
    • WB: A431 cell lysate. IHC-P: Human cervical carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/50.
IP 1/40.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • 配列類似性
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • 翻訳後修飾
      Autophosphorylated on Ser-380, as part of the activation process.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 90 kDa ribosomal protein S6 kinase 1 antibody
      • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
      • dJ590P13.1 antibody
      • EC 2.7.11.1 antibody
      • HU 1 antibody
      • HU1 antibody
      • KS6A1_HUMAN antibody
      • MAP kinase activated protein kinase 1a antibody
      • MAP kinase-activated protein kinase 1a antibody
      • MAPK-activated protein kinase 1a antibody
      • MAPKAP kinase 1a antibody
      • MAPKAPK-1a antibody
      • MAPKAPK1A antibody
      • MGC79981 antibody
      • Mitogen-activated protein kinase-activated protein kinase 1A antibody
      • OTTHUMP00000004113 antibody
      • p90 RSK1 antibody
      • p90-RSK 1 antibody
      • p90rsk antibody
      • p90RSK1 antibody
      • p90S6K antibody
      • pp90RSK1 antibody
      • Ribosomal protein S6 kinase 90kD 1 antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
      • Ribosomal protein S6 kinase alpha 1 antibody
      • Ribosomal protein S6 kinase alpha-1 antibody
      • Ribosomal protein S6 kinase polypeptide 1 antibody
      • Ribosomal S6 kinase 1 antibody
      • RPS6K1 alpha antibody
      • rps6ka antibody
      • Rps6ka1 antibody
      • RSK 1 antibody
      • RSK 1 p90 antibody
      • RSK antibody
      • RSK-1 antibody
      • RSK1 antibody
      • S6K alpha 1 antibody
      • S6K-alpha-1 antibody
      see all

    画像


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 90 kDa


      Exposure time : 16 minutes

      BT549 cells were incubated at 37 °C for 1h with vehicle control (0 μM) and different concentrations of PD 173074 (ab141117) in DMSO. Decreased expression of RSK1 p90 (phospho T359 + S363) (ab32413) correlates with an increase in PD 173074 concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab32413 at 1/5000 dilutionand ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    • All lanes : Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413) at 1/20000 dilution

      Lane 1 : A431 cell lysate untreated.
      Lane 2 : A431 cell lysate treated with EGF.


      Predicted band size : 90 kDa
      Observed band size : 90 kDa
    • Ab32413, at a 1/100 dilution, staining RSK1 p90 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
      Left image : Untreated.
      Right image : Treated with Phosphatase.

    参考文献

    This product has been referenced in:

    See all 5 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Sample
    Human Tissue sections (Human fibromatosis)
    Specification
    Human fibromatosis
    Permeabilization
    Yes - Tween-20
    Fixative
    Paraformaldehyde
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    Verified customer

    投稿 Dec 05 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated
    Sample
    Mouse Tissue sections (mouse liver)
    Specification
    mouse liver
    Permeabilization
    Yes - Tween-20
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Nov 25 2014

    Application
    Western blot
    Loading amount
    75 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Pig Cell lysate - whole cell (PAE cells)
    Specification
    PAE cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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