製品の概要

  • 製品名Anti-RSK1 p90 (phospho S380) antibody [E239]
    RSK1 p90 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E239] to RSK1 p90 (phospho S380)
  • 特異性The antibody only detects RSK1 phosphorylated on Serine 380.
  • アプリケーション適用あり: Dot blot, WB, ICC/IF, IPmore details
    適用なし: Flow Cyt or IHC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Ser380 of human p90RSK1.

  • ポジティブ・コントロール
    • A431 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/1000.
WB 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
ICC/IF 1/250 - 1/500.
IP 1/80.
  • 追加情報Is unsuitable for Flow Cyt or IHC.
  • ターゲット情報

    • 機能Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • 翻訳後修飾Autophosphorylated on Ser-380, as part of the activation process.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 90 kDa ribosomal protein S6 kinase 1 antibody
      • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
      • dJ590P13.1 antibody
      • EC 2.7.11.1 antibody
      • HU 1 antibody
      • HU1 antibody
      • KS6A1_HUMAN antibody
      • MAP kinase activated protein kinase 1a antibody
      • MAP kinase-activated protein kinase 1a antibody
      • MAPK-activated protein kinase 1a antibody
      • MAPKAP kinase 1a antibody
      • MAPKAPK-1a antibody
      • MAPKAPK1A antibody
      • MGC79981 antibody
      • Mitogen-activated protein kinase-activated protein kinase 1A antibody
      • OTTHUMP00000004113 antibody
      • p90 RSK1 antibody
      • p90-RSK 1 antibody
      • p90rsk antibody
      • p90RSK1 antibody
      • p90S6K antibody
      • pp90RSK1 antibody
      • Ribosomal protein S6 kinase 90kD 1 antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
      • Ribosomal protein S6 kinase alpha 1 antibody
      • Ribosomal protein S6 kinase alpha-1 antibody
      • Ribosomal protein S6 kinase polypeptide 1 antibody
      • Ribosomal S6 kinase 1 antibody
      • RPS6K1 alpha antibody
      • rps6ka antibody
      • Rps6ka1 antibody
      • RSK 1 antibody
      • RSK 1 p90 antibody
      • RSK antibody
      • RSK-1 antibody
      • RSK1 antibody
      • S6K alpha 1 antibody
      • S6K-alpha-1 antibody
      see all

    Anti-RSK1 p90 (phospho S380) antibody [E239] 画像

    • Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A431(Human epidermoid carcinoma cell line) cells, treated and non-treated, labeling RSK1 p90 (phospho S380) with Ab32203 at 1/200 dilution followed by Goat anti-Rabbit secondary IgG AlexaFluor®488 (ab150077) secondary antibody at 1/1000 dilution (green).

      The green staining was increased in the EGF(ab9697 100ng/ml, 10min) treated A431 cells when compared with A431 cells without treatment. After LP treatment, the green signaling was obviously decreased.

      For the pan antibody, there was no great difference after EGF (100ng/ml, 10min) or EGF (100ng/ml, 10min) + LP treatment. The data showed mostly Cytoplasm staining.

    • RSK1 p90 (phospho S380) was immunoprecipitated from 10μg A431 (human epidermoid carcinoma) whole cell lysate with ab32203 at 1/20 dilution (0.5μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab32203 at 1/200 dilution (0.54 μg/ml). VeriBlot for IP (HRP) ab131366, was used as secondary antibody at 1/1000 dilution.

      Lane 1 Input: A431(human epidermoid carcinoma) treated with 100ng/ml EGF for 10min whole cell lysate 10μg
      Lane 2 (+): ab32203 + A431(human epidermoid carcinoma) treated with 100ng/ml EGF for 10min whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32203 in A431(human epidermoid carcinoma) treated with 100ng/ml EGF for 10min whole cell lysate

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203) at 1/5000 dilution

      Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
      Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) treated with epidermal growth factor at 100ng/ml for 10 min. Whole cell lysates.
      Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) treated with epidermal growth factor at 100ng/ml for 10 min. Whole cell lysates. Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 83 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Dot blot analysis of RSK1 p90 (phospho S380) phospho peptide (Lane 1) and RSK1 p90 non-phospho peptide (Lane 2) labeling RSK1 p90 (phospho S380) with ab32203 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203) 使用論文

    This product has been referenced in:
    • Smadja-Lamère N  et al. Insulin Activates RSK (p90 Ribosomal S6 Kinase) to Trigger a New Negative Feedback Loop That Regulates Insulin Signaling for Glucose Metabolism. J Biol Chem 288:31165-76 (2013). WB ; Human . Read more (PubMed: 24036112) »
    • Tangredi MM  et al. The C-terminal kinase and ERK-binding domains of Drosophila S6KII (RSK) are required for phosphorylation of the protein and modulation of circadian behavior. J Biol Chem 287:16748-58 (2012). WB ; Drosophila melanogaster . Read more (PubMed: 22447936) »

    See all 8 Publications for this product

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