Rabbit polyclonal to RSK1 p90 (phospho S364)
Synthetic phosphopeptide corresponding to amino acid residues surrounding S364 of human RSK1 p90 conjugated to KLH
Human Hepatocarcinoma tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Protein G purified
ab59997 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain. Contains 2 protein kinase domains.
Autophosphorylated on Ser-380, as part of the activation process.
Information by UniProt
90 kDa ribosomal protein S6 kinase 1 antibody
dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RSK1 p90 (phospho S364) antibody (ab59997)
ab59997 at 1/50-1/100 dilution staining RSK1 p90 in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining
has not yet been referenced specifically in any publications.
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