Anti-Rsk 2 / MAPKAP Kinase 1b 抗体 [Y82] (ab32062)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y82] to Rsk 2 / MAPKAP Kinase 1b
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82]
Rsk 2 / MAPKAP Kinase 1b 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y82] to Rsk 2 / MAPKAP Kinase 1b -
由来種
Rabbit -
特異性
This antibody is specific for human Rsk 2 / MAPKAP Kinase 1b. -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human Rsk 2/ MAPKAP Kinase 1b (C terminal). The exact sequence is proprietary.
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エピトープ
ab32062 reacts with an epitope located in the C terminal region of Rsk 2 / MAPKAP Kinase 1b. -
ポジティブ・コントロール
- Flow Cyt (intra): MCF7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y82 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32062の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000. Predicted molecular weight: 90 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 90 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. -
組織特異性
Expressed in many tissues, highest levels in skeletal muscle. -
関連疾患
Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻訳後修飾
Autophosphorylated on Ser-386, as part of the activation process.
Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369. - Information by UniProt
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参照データベース
- Entrez Gene: 6197 Human
- Entrez Gene: 110651 Mouse
- Entrez Gene: 501560 Rat
- Omim: 300075 Human
- SwissProt: P51812 Human
- SwissProt: P18654 Mouse
- Unigene: 445387 Human
- Unigene: 328476 Mouse
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別名
- 90 kDa ribosomal protein S6 kinase 3 antibody
- CLS antibody
- HU 3 antibody
see all
画像
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Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
Lane 3: MCF7 cell lysate (40 µg)
Lane 4: HeLa cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32062 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.ab32062 was shown to recognize Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used, along with additional cross-reactive bands. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32062 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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ab32062 at a 1:250 - 1:500 dilution staining Rsk 2 / MAPKAP Kinase 1b in human urinary bladder carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Overlay histogram showing MCF7 cells stained with ab32062 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32062, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062) at 1/1000 dilution + MCF7 cell lysate
Predicted band size: 90 kDa
Observed band size: 90 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab32062 は 2 報の論文で使用されています。
- Qian X et al. Therapeutic Effect of Idebenone on Rats with Vascular Dementia via the MicroRNA-216a/RSK2/NF-?B Axis. Neuropsychiatr Dis Treat 17:533-543 (2021). PubMed: 33628024
- Li Y et al. Plantamajoside modulates the proliferation, stemness, and apoptosis of lung carcinoma via restraining p38MAPK and AKT phosphorylation. Transl Cancer Res 9:3828-3841 (2020). PubMed: 35117750