遺伝子ノックアウト細胞株 検証済
リコンビナント
RabMAb

Anti-Rsk 2 / MAPKAP Kinase 1b 抗体 [Y82] (ab32062)

製品の概要

  • 製品名
    Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82]
    Rsk 2 / MAPKAP Kinase 1b 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y82] to Rsk 2 / MAPKAP Kinase 1b
  • 特異性
    This antibody is specific for human Rsk 2 / MAPKAP Kinase 1b.
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cyt, IPmore details
    適用なし: ICC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    corresponding to Human Rsk 2/ MAPKAP Kinase 1b (C terminal).

  • エピトープ
    ab32062 reacts with an epitope located in the C terminal region of Rsk 2 / MAPKAP Kinase 1b.
  • ポジティブ・コントロール
    • MCF7 cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C prior to reconstitution. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    Y82
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab32062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 90 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
  • 追加情報
    Is unsuitable for ICC.
  • ターゲット情報

    • 機能
      Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • 組織特異性
      Expressed in many tissues, highest levels in skeletal muscle.
    • 関連疾患
      Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
    • 配列類似性
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • 翻訳後修飾
      Autophosphorylated on Ser-386, as part of the activation process.
      Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 90 kDa ribosomal protein S6 kinase 3 antibody
      • CLS antibody
      • HU 3 antibody
      • HU2 antibody
      • HU3 antibody
      • Insulin stimulated protein kinase 1 antibody
      • Insulin-stimulated protein kinase 1 antibody
      • ISPK-1 antibody
      • ISPK1 antibody
      • KS6A3_HUMAN antibody
      • MAP kinase activated protein kinase 1b antibody
      • MAP kinase-activated protein kinase 1b antibody
      • MAPK activated protein kinase 1b antibody
      • MAPK-activated protein kinase 1b antibody
      • MAPKAP kinase 1b antibody
      • MAPKAPK 1b antibody
      • MAPKAPK-1b antibody
      • MAPKAPK1B antibody
      • Mental retardation, X linked 19 antibody
      • MRX19 antibody
      • OTTHUMP00000023036 antibody
      • p90 RSK2 antibody
      • p90 RSK3 antibody
      • p90-RSK 3 antibody
      • p90RSK3 antibody
      • pp90RSK2 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 3 antibody
      • Ribosomal protein S6 kinase alpha 3 antibody
      • Ribosomal protein S6 kinase alpha-3 antibody
      • Ribosomal protein s6 kinase ii alpha 2 antibody
      • Ribosomal S6 kinase 2 antibody
      • Rps6ka3 antibody
      • RSK antibody
      • RSK-2 antibody
      • RSK2 antibody
      • S6 kinase 2 antibody
      • S6K alpha3 antibody
      • S6K-alpha-3 antibody
      see all

    画像



    • Predicted band size : 90 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
      Lane 3: MCF7 cell lysate (40 µg)
      Lane 4: HeLa cell lysate (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32062 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32062 was shown to recognize Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used, along with additional cross-reactive bands. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32062 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • ab32062 at a 1:250 - 1:500 dilution staining Rsk 2 / MAPKAP Kinase 1b in human urinary bladder carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
    • Overlay histogram showing MCF7 cells stained with ab32062 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32062, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y82] (ab32062) at 1/1000 dilution + MCF7 cell lysate.

      Predicted band size : 90 kDa
      Observed band size : 90 kDa

    参考文献

    ab32062 has not yet been referenced specifically in any publications.

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab32062.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録