Rsk 2 / MAPKAP Kinase 1b overexpression 293T lysate (whole cell) (ab94181)

製品の概要

  • 製品名Rsk 2 / MAPKAP Kinase 1b overexpression 293T lysate (whole cell)
  • 特記事項ab94181 is a 293T cell transfected lysate in which Human Rsk 2 / MAPKAP Kinase 1b has been transiently over-expressed using a pCMV-Rsk 2 / MAPKAP Kinase 1b plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Function: Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Tissue specificity: Expressed in many tissues, highest levels in skeletal muscle. Disease: Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 protein kinase domains. PTM: Autophosphorylated on Ser-386, as part of the activation process. Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab94181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

Rsk 2 / MAPKAP Kinase 1b overexpression 293T lysate (whole cell) 画像

  • ab94181 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Rsk 2 / MAPKAP Kinase 1b antibody (ab76886) at 1/500 dilution

    Lane 1 : Rsk 2 / MAPKAP Kinase 1b overexpression 293T lysate (whole cell) (ab94181)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Rsk 2 / MAPKAP Kinase 1b overexpression 293T lysate (whole cell) (ab94181) 使用論文

ab94181 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"