製品の概要

  • 製品名Anti-RSG1 antibody
    RSG1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RSG1
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KFDQYMHTDV PERDLTAFRQ AWELPLLRVK SVPGRRLADG RTLDGRAGLA DVAHILNGLA EQLWHQDQVA AGLLPNPPES APE, corresponding to amino acids 176-258 of Human RSG1 (Q9BU20).

  • ポジティブ・コントロール
    • Human liver tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab121990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

ターゲット情報

  • 機能Potential effector of the planar cell polarity signaling pathway. Plays a role in targeted membrane trafficking most probably at the level of vesicle fusion with membranes. Involved in cilium biogenesis by regulating the transport of cargo proteins to the basal body and to the apical tips of cilia. More generally involved in exocytosis in secretory cells.
  • 配列類似性Belongs to the small GTPase superfamily. Rab family.
  • 細胞内局在Cytoplasm > cytoskeleton > cilium basal body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C1orf89 antibody
    • Miro domain containing protein C1orf89 antibody
    • Rem/Rab Similar GTPase 1 antibody
    • REM2- and Rab-like small GTPase 1 antibody
    • RP4-733M16.4 antibody
    • Rsg1 antibody
    • RSG1_HUMAN antibody
    see all

Anti-RSG1 antibody 画像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei but not nucleoli. Recommended concentration of ab121990 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121990, at 1/40 dilution, staining RSG1 in Paraffin-embedded Human liver tissue by Immunohistochemistry.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

Anti-RSG1 antibody (ab121990) 使用論文

ab121990 has not yet been referenced specifically in any publications.

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