Anti-RSF1 抗体 [EPR3749(2)] (ab109002)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3749(2)] to RSF1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RSF1 antibody [EPR3749(2)]
RSF1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3749(2)] to RSF1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Jurkat, HEK-293T and HeLa cell lysates. IHC-P: Human colon, endometrial adenocarcinoma and tonsil tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3749(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109002の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/80.
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ICC/IF |
1/100.
For unpurified use at 1/500-1/1000. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 164 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. See IHC antigen retrieval protocols. For unpurified use at 1/250- 1/500.
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特記事項 |
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Flow Cyt (Intra)
1/80. |
ICC/IF
1/100. For unpurified use at 1/500-1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 164 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. See IHC antigen retrieval protocols. For unpurified use at 1/250- 1/500.
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ターゲット情報
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関連性
RSF1 is involved in transcription repression when recruited to DNA via the DNA binding of GAL4. It is also involved in chromatin remodeling and spacing when associated with SNF2H and transcription coactivation when associated with hepatitis B virus X protein (HBX). The RSF1 gene is localized to the long arm of human chromosome 11 between q13.4 and q14.1. This region is amplified and rearranged in many tumors, indicating that it plays a role in tumorigenesis similar to that of other PHD-containing proteins. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 51773 Human
- Omim: 608522 Human
- SwissProt: Q96T23 Human
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別名
- HBV pX associated protein 8 antibody
- Hepatitis B virus x associated protein antibody
- HXBAP antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with Purified ab109002 at 1:100 (8.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with purified ab109002 at 1/80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-RSF1 antibody [EPR3749(2)] (ab109002) at 1/1000 dilution (unpurified)
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 164 kDa -
Paraffin-embedded human colon tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Paraffin-embedded human endometrial adenocarcinoma tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Paraffin-embedded human tonsil tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab109002 は 10 報の論文で使用されています。
- Zhao Y & Hong L lncRNA-PRLB Confers Paclitaxel Resistance of Ovarian Cancer Cells by Regulating RSF1/NF-?B Signaling Pathway. Cancer Biother Radiopharm 36:202-210 (2021). PubMed: 33156701
- Stefos GC et al. aniFOUND: analysing the associated proteome and genomic landscape of the repaired nascent non-replicative chromatin. Nucleic Acids Res 49:e64 (2021). PubMed: 33693861
- Höflmayer D et al. High RSF1 protein expression is an independent prognostic feature in prostate cancer. Acta Oncol 59:268-273 (2020). PubMed: 31687881
- Yang L et al. LncRNA NEAT1 Promotes the Progression of Gastric Cancer Through Modifying the miR-1224-5p/RSF1 Signaling Axis. Cancer Manag Res 12:11845-11855 (2020). PubMed: 33244266
- Liu Y et al. Effects of RSF-1 on proliferation and apoptosis of breast cancer cells. Oncol Lett 16:4279-4284 (2018). PubMed: 30214561
- Lee HS et al. The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation. Nat Commun 9:3848 (2018). PubMed: 30242288
- Min S et al. Post-Translational Regulation of the RSF1 Chromatin Remodeler under DNA Damage. Mol Cells 41:127-133 (2018). WB . PubMed: 29385673
- Zhao X et al. MicroRNA-154 as a prognostic factor in bladder cancer inhibits cellular malignancy by targeting RSF1 and RUNX2. Oncol Rep 38:2727-2734 (2017). PubMed: 29048677
- Lee HS et al. The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores. Nat Commun 6:7904 (2015). ICC . PubMed: 26259146
- Ren J et al. Overexpression of Rsf-1 correlates with pathological type, p53 status and survival in primary breast cancer. Int J Clin Exp Pathol 7:5595-608 (2014). IHC ; Human . PubMed: 25337201