製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RRP7A
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to an internal region between amino acids 47-160 of the Human RRP7A protein (Q9Y3A4).

  • ポジティブ・コントロール
    • Human RT-4, EFO-21, A-431, and Tonsil cell lysates. Human lateral ventricle tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

ターゲット情報

  • 配列類似性
    Belongs to the RRP7 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110014J01Rik antibody
    • AA408146 antibody
    • BK126B4.3, antibody
    • CGI 96 antibody
    • CGI-96 antibody
    • CTA-126B4.5 antibody
    • Gastric cancer antigen Zg14 antibody
    • Kheg1 antibody
    • MGC150422 antibody
    • MGC150423 antibody
    • RGD1311547 antibody
    • Ribosomal RNA-processing protein 7 homolog A antibody
    • Rrp7a antibody
    • RRP7A_HUMAN antibody
    see all

画像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab121142 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical staining of Human lateral ventricle with ab121142 1/150 dilution. The staining shows nucleolar positivity in neuronal cells and nuclear positivity in glial cells.
  • All lanes : Anti-RRP7A antibody (ab121142) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : EFO-21 cell lysate
    Lane 3 : A-431 cell lysate
    Lane 4 : Liver cell lysate
    Lane 5 : Tonsil cell lysate

参考文献

ab121142 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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