Anti-RRM2 抗体 [1E1] (ab57653)
Key features and details
- Mouse monoclonal [1E1] to RRM2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-RRM2 antibody [1E1]
RRM2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1E1] to RRM2 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 1-111 of Human RRM2
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特記事項
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
1E1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab57653の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. -
パスウェイ
Genetic information processing; DNA replication. -
配列類似性
Belongs to the ribonucleoside diphosphate reductase small chain family. -
翻訳後修飾
Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 6241 Human
- Omim: 180390 Human
- SwissProt: P31350 Human
- Unigene: 226390 Human
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別名
- cb111 antibody
- chunp6884 antibody
- etID309896.20 antibody
see all
画像
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Anti-RRM2 antibody [1E1] (ab57653) at 1 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 45 kDaThis image was generated using the ascites version of the product.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RRM2 (green) using ab57653 at 10 µg/ml in ICC/IF.
This image was generated using the ascites version of the product.
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RRM2 antibody (ab57653) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach.
This image was generated using the ascites version of the product.
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RRM2 antibody (ab57653) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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ICC/IF image of ab57653 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57653, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product. -
Overlay histogram showing HepG2 cells stained with ab57653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57653, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (36)
ab57653 は 36 報の論文で使用されています。
- Song X et al. Construction of a Novel Ferroptosis-Related Gene Signature for Predicting Survival of Patients With Lung Adenocarcinoma. Front Oncol 12:810526 (2022). PubMed: 35311093
- Liu Y et al. MiR-200c-3p and miR-485-5p overexpression elevates cisplatin sensitivity and suppresses the malignant phenotypes of non-small cell lung cancer cells through targeting RRM2. Thorac Cancer 13:1974-1985 (2022). PubMed: 35599447
- Bothou C et al. Novel Insights into the Molecular Regulation of Ribonucleotide Reductase in Adrenocortical Carcinoma Treatment. Cancers (Basel) 13:N/A (2021). PubMed: 34439352
- Zhuang S et al. RRM2 elicits the metastatic potential of breast cancer cells by regulating cell invasion, migration and VEGF expression via the PI3K/AKT signaling. Oncol Lett 19:3349-3355 (2020). PubMed: 32256828
- Krajewski A et al. Cyclin F is involved in response to cisplatin treatment in melanoma cell lines. Oncol Rep 43:765-772 (2020). PubMed: 32020229