製品の概要

  • 製品名Anti-RRAS2 antibody
    RRAS2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to RRAS2
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-205 of Human RRAS2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion.
  • 組織特異性Ubiquitously present in all tissues examined, with the highest levels in heart, placenta, and skeletal muscle. Moderate levels in lung and liver; low levels in brain, kidney, and pancreas.
  • 関連疾患Ovarian cancer
  • 配列類似性Belongs to the small GTPase superfamily. Ras family.
  • 翻訳後修飾May be post-translationally modified by both palmitoylation and polyisoprenylation.
  • 細胞内局在Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).
  • Information by UniProt
  • 参照データベース
  • 別名
    • C86394 antibody
    • Oncogene RRAS2 antibody
    • Oncogene TC21 antibody
    • Ras like protein TC21 antibody
    • Ras related protein R Ras2 antibody
    • Ras-like protein TC21 antibody
    • Ras-related protein R-Ras2 antibody
    • Related RAS viral (r ras) oncogene homolog 2 antibody
    • Related RAS viral oncogene homolog 2 antibody
    • RRAS 2 antibody
    • RRAS2 antibody
    • RRAS2_HUMAN antibody
    • TC 21 antibody
    • TC21 antibody
    • Teratocarcinoma oncogene antibody
    • Teratocarcinoma oncogene TC21 antibody
    see all

Anti-RRAS2 antibody 画像

  • Overlay histogram showing A431 cells stained with ab56859 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56859, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • RRAS2 antibody (ab56859) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human dysgerminoma tissue.
  • RRAS2 antibody (ab56859) at 1ug/lane + A-431 cell lysate at 25ug/lane.

Anti-RRAS2 antibody (ab56859) 使用論文

This product has been referenced in:
  • Dahiya N  et al. Claudin-7 is frequently overexpressed in ovarian cancer and promotes invasion. PLoS One 6:e22119 (2011). WB ; Human . Read more (PubMed: 21789222) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab56859.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"