製品の概要

  • 製品名Anti-RRAGD antibody
    RRAGD 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to RRAGD
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human RRAGD protein (NP_067067.1, 1 a.a. ~ 400 a.a).

  • ポジティブ・コントロール
    • RRAGD transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.

ターゲット情報

  • 機能Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
  • 配列類似性Belongs to the GTR/RAG GTP-binding protein family.
  • 細胞内局在Cytoplasm. Nucleus. Lysosome. Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA11D8.2.1 antibody
    • DKFZp761H171 antibody
    • FLJ44176 antibody
    • OTTHUMP00000016853 antibody
    • Rag D antibody
    • Rag D protein antibody
    • RagD antibody
    • RagDRRAGD antibody
    • Ras related GTP binding D antibody
    • Ras-related GTP binding protein D antibody
    • Ras-related GTP-binding protein D antibody
    • RRAGD antibody
    • RRAGD_HUMAN antibody
    see all

Anti-RRAGD antibody 画像

  • All lanes : Anti-RRAGD antibody (ab73090) at 1/500 dilution

    Lane 1 : RRAGD transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 46 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

Anti-RRAGD antibody (ab73090) 使用論文

ab73090 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa, 293T)
Loading amount 15 µg
Specification HeLa, 293T
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"