Anti-RRAGC 抗体 [2938C3a] (ab76577)
Key features and details
- Mouse monoclonal [2938C3a] to RRAGC
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG2b
製品の概要
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製品名
Anti-RRAGC antibody [2938C3a]
RRAGC 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2938C3a] to RRAGC -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment comprising of 50-200 amino acids from near the N terminus of Human RRAGC (NM_022157).
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ポジティブ・コントロール
- Recombinant fragment of human RRAGC.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab76577 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
2938C3a -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76577の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 44 kDa).
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特記事項 |
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WB
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 44 kDa). |
ターゲット情報
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機能
Has guanine nucleotide-binding activity but weak intrinsic GTPase activity. Probably required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. -
配列類似性
Belongs to the GTR/RAG GTP-binding protein family. -
細胞内局在
Cytoplasm. Nucleus. Lysosome. Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA. - Information by UniProt
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別名
- AU041672 antibody
- FLJ13311 antibody
- GTPase interacting protein 2 antibody
see all
画像
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Anti-RRAGC antibody [2938C3a] (ab76577) at 2 µg/ml + immunising recombinant protein
Predicted band size: 44 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab76577 は論文での使用が確認できていません。