製品の概要

  • 製品名Anti-RRAGA + RRAGB antibody
    RRAGA + RRAGB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RRAGA + RRAGB
  • アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human RRAGA + RRAGB.

    (Peptide available as ab91427.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following while cell lysates: HeLa; Jurkat; HEK293; Raji; SHSY-5Y.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).

ターゲット情報

  • 関連性Involved in the RCC1/Ran-GTPase pathway. RRAGA may play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. biquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.
  • 細胞内局在Cytoplasmic and Nuclear: RRAGA may shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state and colocalizes in vivo with adenovirus E3-14.7K. RRAGB is cytoplasmic.
  • 参照データベース
  • 別名
    • Adenovirus E3 14.7 kDa-interacting protein 1 antibody
    • FIP1 antibody
    • Rag A antibody
    • RAg B antibody
    • RagA antibody
    • RAGB antibody
    • Ras related GTP binding protein A antibody
    • Ras-related GTP binding protein B antibody
    see all

Anti-RRAGA + RRAGB antibody 画像

  • All lanes : Anti-RRAGA + RRAGB antibody (ab91062) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg/ml per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • ICC/IF image of ab91062 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91062, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • IHC image of RRAGA + RRAGB staining in human normal formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91062, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-RRAGA + RRAGB antibody (ab91062) 使用論文

ab91062 has not yet been referenced specifically in any publications.

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