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RabMAb

Anti-RPS6 (phospho S235) 抗体 [EP1338(2)Y] - Ribosome Marker (ab80158)

製品の概要

  • 製品名
    Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker
    RPS6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1338(2)Y] to RPS6 (phospho S235) - Ribosome Marker
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RPS6.

  • ポジティブ・コントロール
    • Human breast adenocarcinoma tissue; HeLa cell lysate, untreated or treated with Calyculin A.
  • 特記事項

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP1338(2)Y
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/200000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP 1/30.
IHC-P Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能
      May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
    • 配列類似性
      Belongs to the ribosomal protein S6e family.
    • 翻訳後修飾
      Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 40S ribosomal protein S6 antibody
      • Air8 antibody
      • NP33 antibody
      • Phosphoprotein NP33 antibody
      • Pp30 antibody
      • Ribosomal protein S6 antibody
      • RP S6 antibody
      • rps6 antibody
      • RS6 antibody
      • RS6_HUMAN antibody
      • S6 antibody
      • S6 Ribosomal Protein antibody
      see all

    Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker 画像

    • ab80158 at 1/100-1/250 dilution staining RPS6 in human breast adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • All lanes : Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158) at 1/1000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h. Whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h, then 20% FBS incubated for 90 minutes. Whole cell lysates
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) serum starved for 24 h, then 20% FBS incubated for 90 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 30 kDa
      Observed band size : 30 kDa


      Exposure time : 1 minute

      Blocking and diluting  buffer and concentration: 5% NFDM/TBST.

    • Dot Blot analysis of -

      Lane 1: Rb (pS235) phospho peptide

      Lane 2: Rb (pS236) phospho peptide

      Lane 3: Rb (pS235 & pS236) phospho peptide

      Lane 4: Rb non-phospho peptide

      5% NFDM/TBST was used as the diluting and blocking buffer.

      ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

    • All lanes : Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158) at 1/200000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 30 kDa
      Observed band size : 30 kDa

    Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - Ribosome Marker (ab80158) 使用論文

    This product has been referenced in:
    • Chuang JH  et al. Differentiation of glutamatergic neurons from mouse embryonic stem cells requires raptor S6K signaling. Stem Cell Res 11:1117-1128 (2013). Mouse . Read more (PubMed: 23988668) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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