製品の概要

  • 製品名Anti-RPS6 antibody
    RPS6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RPS6
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human, Caenorhabditis elegans
    交差が予測される動物種: Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Reptiles, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 100 and 150 of human RPS6. ( NP_001001.2).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and mouse NIH3T3 cells. IHC-P: FFPE human skin tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

Anti-RPS6 antibody 画像

  • Anti-RPS6 antibody (ab70227) at 1/1000 dilution + Caenorhabditis elegans whole adult tissue lysate at 200 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa (possible non-specific binding),26 kDa (possible non-specific binding),40 kDa (possible non-specific binding).

    Exposure time : 40 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • IHC image of RPS6 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70227, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-RPS6 antibody (ab70227) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 30 kDa
    Observed band size : 30 kDa


    Exposure time : 30 seconds
  • Detection of Human RPS6 by Immunoprecipitation. Samples: A) Whole cell lysate from HeLa cells (1 mg/IP; 1/4 of IP loaded) using affinity purified rabbit anti-RPS6 antibody ab70227 at 3 µg/mg lysate for IP and at 0.04 µg/ml for subequent WB detection. Second lane represents IgG IP control.

Anti-RPS6 antibody (ab70227) 使用論文

This product has been referenced in:
  • Hagner PR  et al. Ribosomal protein S6 is highly expressed in non-Hodgkin lymphoma and associates with mRNA containing a 5' terminal oligopyrimidine tract. Oncogene : (2010). IP ; Human . Read more (PubMed: 21102526) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 200 µg
Gel Running Conditions Reduced Denaturing
Sample Caenorhabditis elegans Tissue lysate - whole (whole adult)
Specification whole adult
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Mar 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"