製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RPS4Y1
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthesized peptide derived from C-terminal of human RPS4Y1.

  • ポジティブ・コントロール
    • Extracts from COLO cells, HeLa cells and HUVEC cells; HUVEC cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 29 kDa.
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 配列類似性
    Belongs to the ribosomal protein S4e family.
    Contains 1 S4 RNA-binding domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 40S ribosomal protein S4 antibody
    • 40S ribosomal protein S4, Y antibody
    • 40S ribosomal protein S4, Y isoform 1 antibody
    • MGC119100 antibody
    • MGC5070 antibody
    • ribosomal protein S4, Y linked 1 antibody
    • ribosomal protein S4, Y linked antibody
    • ribosomal protein S4, Y-linked 1 antibody
    • ribosomal protein S4Y antibody
    • RPS4Y antibody
    • RPS4Y1 antibody
    • RS4Y1_HUMAN antibody
    • S4 antibody
    • Y isoform 1 antibody
    see all

画像

  • All lanes : Anti-RPS4Y1 antibody (ab74709) at 1/500 dilution

    Lane 1 : Extracts from COLO cells with no peptide
    Lane 2 : Extracts from HeLa cells with no peptide
    Lane 3 : Extracts from HUVEC cells with no peptide
    Lane 4 : Extracts from HUVEC cells with peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HuvEc cells, using ab74709 at a dilution of 1/500. Left-hand panel: untreated sample; right-hand panel: with immunising peptide.

参考文献

ab74709 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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