製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RPS3A
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Pig, Zebrafish
  • 免疫原

    Recombinant fragment corresponding to a region within amino acids 14-262 of Human RPS3A (NP_000997).

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji whole cell lysates; HeLa cells.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

Anti-RPS3A antibody 画像

  • All lanes : Anti-RPS3A antibody (ab96534) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa S3 whole cell lysate
    Lane 5 : Hep G2 whole cell lysate
    Lane 6 : Molt4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 30 kDa
  • ab96534, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

Anti-RPS3A antibody (ab96534) 使用論文

ab96534 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Nov 30 2012

Thank you for your enquiry.

We have not tested if other sub-families could also be detected with the product but according to amino acid sequence homology, these are the percentage of cross reactivity.




http://www.nc...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Specification
Prostate
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

投稿 Oct 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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