製品の概要

  • 製品名Anti-RPS3 antibody
    RPS3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RPS3
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Pig, Xenopus laevis, Non Human Primates, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 RPS3.

    (Peptide available as ab86941.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Mouse Liver Tissue; Mouse Kidney Tissue; Rat Liver Tissue; Rat Lung Tissue; HepG2 Whole Cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 27 kDa).

ターゲット情報

Anti-RPS3 antibody 画像

  • All lanes : Anti-RPS3 antibody (ab77772) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Lung (Rat) Tissue Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab77772 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77772, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and Hek293 cells at 5µg/ml, and in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.
  • IHC image of RPS3 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77772, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-RPS3 antibody (ab77772) 使用論文

ab77772 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your answer.

I would recommend to include a positive control in the experiment, where the expression of RPS3 is known to be expressed, such as liver tissue or cells (HepG2). This way there will be no ambiguity on the results. Read More

Thank you for your inquiry.

We have tested the ab77772 in immunocytochemistry and can therefore guarantee that it will work in this application. As I am not an expert in stem cell biology, I would recommend to check the literature to see whet...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for your enquiry.

For ab77772, I am afraid that we have not tested for isoform detection. I did a sequence comparison with RPS3A protein sequence and found that the homology is only 12%. Therefore, it is unlikely that there will be...

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