製品の概要

  • 製品名Anti-RPS20 antibody
    RPS20 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RPS20
  • アプリケーション適用あり: WB, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide derived from an internal sequence of human RPS20.

  • ポジティブ・コントロール
    • HUVEC and MCF-7 cell extracts. A549 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 13 kDa.
ICC/IF 1/500 - 1/1000.
ELISA 1/40000.

ターゲット情報

Anti-RPS20 antibody 画像

  • All lanes : Anti-RPS20 antibody (ab74700) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells (5-30µg)
    Lane 2 : Extracts from MCF-7 cells (5-30µg)
    Lane 3 : Extracts from HUVEC cells (5-30µg), plus immunising peptide (5-10µg)


    Predicted band size : 13 kDa
    Observed band size : <19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescent staining of A549 cells, using ab74700 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).

Anti-RPS20 antibody (ab74700) 使用論文

This product has been referenced in:
  • Daftuar L  et al. Ribosomal proteins RPL37, RPS15 and RPS20 regulate the Mdm2-p53-MdmX network. PLoS One 8:e68667 (2013). WB . Read more (PubMed: 23874713) »
  • Zuccotti M  et al. Gatekeeper of pluripotency: A common Oct4 transcriptional network operates in mouse eggs and embryonic stem cells. BMC Genomics 12:345 (2011). ICC/IF ; Mouse . Read more (PubMed: 21729306) »

See all 2 Publications for this product

Product Wall

Thank you for calling us yesterday and sorry about the delay in getting back to you.
I have had a look into the anti-LC3B antibodies we have which may be suitable for the experiment you are hoping to perform. I could not find any goat anti-LC3B an...

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There was no significant similarity found between the immunogen sequence and the sequence that you provided. Therefore I would not recommend this antibody to you. We do not have any other RPS20 antibodies in our catalogue that I can recommend but I wo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"