製品の概要

  • 製品名Anti-Rpn8 antibody
  • 製品の詳細
    Mouse polyclonal to Rpn8
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Recombinant fragment: LGTPDDDEID VENHDRINIS NNLQKALTVK TNDELMVIYI SNLVRSIIAF DDLIENKIQN KKIQEQRVKD KQSKVSDDSE SESGDKEATA PLIQRKNKKN , corresponding to amino acids 239-339 of Saccharomyces cerevisiae Rpn8

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab43730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 38 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性Rpn8 is an essential, non-ATPase regulatory subunit of the 26S proteasome. It has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p.
  • 参照データベース
    • 別名
      • 26S proteasome regulatory subunit RPN8 antibody
      • YOR261C antibody

    Anti-Rpn8 antibody 画像

    • All lanes : Anti-Rpn8 antibody (ab43730) at 1/1000 dilution

      Lane 1 : a total protein extract from E coli with 50ng of a Tagged irrelevant antigen (Negative control)
      Lane 2 : a total protein extract from E coli with 50ng of the antigen (Tagged fusion protein)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) HRP at 1/5000 dilution

      Predicted band size : 38 kDa

    Anti-Rpn8 antibody (ab43730) 使用論文

    ab43730 has not yet been referenced specifically in any publications.

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    Given that alignment result, it is unlikely the antibody will react with fission yeast Rpn8. We predict cross-reactivity when the identity is 85% or more.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"