All lanes : Anti-RPLP0 antibody (ab101279) at 0.1 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg Lane 2 : Hela whole cell lysate at 15 µg Lane 3 : Hela whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Exposure time: 3 minutes
Immunoprecipitation - RPLP0 antibody (ab101279)
Detection of RPLP0 by Western Blot of Immunprecipitate.
ab101279, at 1 µg/ml, staining RPLP0 in HeLa whole cell lysate immunoprecipitated using ab101279 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 30 seconds.
Aviner R et al. Proteomic analysis of polyribosomes identifies splicing factors as potential regulators of translation during mitosis. Nucleic Acids Res45:5945-5957 (2017).
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