製品の概要

  • 製品名Anti-RPL9 antibody
    RPL9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RPL9
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    A region within synthetic peptide: LILEGNDIEL VSNSAALIQQ ATTVKNKDIR KFLDGIYVSE KGTVQQADE, corresponding to C terminal amino acids 144-192 of Human RPL9

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

ターゲット情報

Anti-RPL9 antibody 画像

  • Lane 1 : Molecular weight markers
    Lane 2 : Anti-RPL9 antibody (ab50384) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 21 kDa
    Observed band size : 21 kDa

Anti-RPL9 antibody (ab50384) 使用論文

ab50384 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Drosophila melanogaster Cell lysate - whole cell (S2 Drosophila cells)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 200000 cells
Specification S2 Drosophila cells
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Gerard Rimesso

Verified customer

投稿 Jul 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"