Anti-RPL39 抗体 (ab74740)
Key features and details
- Rabbit polyclonal to RPL39
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPL39 antibody -
製品の詳細
Rabbit polyclonal to RPL39 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HepG2 and COLO cell extracts ICC/IF: A549 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab74740 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74740の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 7 kDa.
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ICC/IF |
1/500 - 1/1000.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 7 kDa. |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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関連性
RPL39 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the S39E family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein is the smallest, and one of the most basic, proteins of the ribosome. This gene is cotranscribed with the U69 small nucleolar RNA gene, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. -
参照データベース
- Entrez Gene: 6170 Human
- Entrez Gene: 67248 Mouse
- Entrez Gene: 25347 Rat
- Omim: 601904 Human
- SwissProt: P62891 Human
- SwissProt: P62892 Mouse
- SwissProt: P62893 Rat
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別名
- 2810465O16Rik antibody
- 60S ribosomal protein L39 antibody
- OTTHUMP00000023908 antibody
see all
画像
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All lanes : Anti-RPL39 antibody (ab74740) at 1/500 dilution
Lane 1 : HepG2 cell extracts
Lane 2 : COLO cell extracts
Lane 3 : COLO cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 7 kDa
Observed band size: 7 kDa -
ab74740 at 1/500 dilution staining RPL39 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab74740 は 2 報の論文で使用されています。
- Shigeoka T et al. On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons. Cell Rep 29:3605-3619.e10 (2019). PubMed: 31825839
- Dave B et al. Targeting RPL39 and MLF2 reduces tumor initiation and metastasis in breast cancer by inhibiting nitric oxide synthase signaling. Proc Natl Acad Sci U S A 111:8838-43 (2014). Human . PubMed: 24876273