製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RPL35
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant protein corresponding to amino acids 2-122 of Human RPL35 (P42766).

  • ポジティブ・コントロール
    • Human pancreas tissue, Human cell line U-2 OS

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/250. Predicted molecular weight: 15 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

ターゲット情報

画像

  • Anti-RPL35 antibody (ab121244) + MCF7 cell lysate

    Predicted band size : 15 kDa
  • Immunohistochemical staining of paraffin embedded Human pancreas, using 1/25 ab121244, shows strong cytoplasmic positivity in exocrine glandular cells.
  • Immunofluorescent staining with 4 µg/ml ab121244 of Human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green.

参考文献

ab121244 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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