Anti-RPL35 抗体 (ab121244)
Key features and details
- Rabbit polyclonal to RPL35
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPL35 antibody
RPL35 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPL35 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant protein corresponding to amino acids 2-122 of Human RPL35 (P42766).
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ポジティブ・コントロール
- IHC-P: Human pancreas, skin, cerebral cortex, colon and fallopian tube tissue. ICC/IF: Human cell line U-2 OS WB: MCF7 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121244の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 15 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 15 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Belongs to the ribosomal protein L29P family. - Information by UniProt
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参照データベース
- Entrez Gene: 11224 Human
- SwissProt: P42766 Human
- Unigene: 182825 Human
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別名
- 60S ribosomal protein L35 antibody
- L35 antibody
- Ribosomal protein L35 antibody
see all
画像
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Anti-RPL35 antibody (ab121244) at 0.4 µg/ml + MCF7 cell lysate
Predicted band size: 15 kDa -
Immunohistochemical analysis of human cerebral fallopian tube labeling rpl35 with ab121244. The data shows moderate cytoplasmic positivity in glandular cells.
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Immunofluorescent staining with 4 µg/ml ab121244 of Human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green.
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Immunohistochemical analysis of human cerebral cortex tissue labeling rpl35 with ab121244. The data shows moderate cytoplasmic positivity in neurons.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling RPL35 with ab121244 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human skin tissue labeling rpl35 with ab121244. The data shows weak staining in squamous epithelial cells.
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Immunohistochemical analysis of human cerebral colon labeling rpl35 with ab121244. The data shows moderate cytoplasmic positivity in glandular cells.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab121244 は 1 報の論文で使用されています。
- Liu PY et al. The long noncoding RNA lncNB1 promotes tumorigenesis by interacting with ribosomal protein RPL35. Nat Commun 10:5026 (2019). PubMed: 31690716