製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RPL34
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRG, corresponding to N terminal amino acids 1-55 of Human RPL34

  • ポジティブ・コントロール
    • Human pancreas tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.

ターゲット情報

  • 関連性
    Belongs to the ribosomal protein L34e family.
  • 細胞内局在
    cytosolic large ribosomal subunit
  • 参照データベース
  • 別名
    • 60S ribosomal protein L34 antibody
    • L34 antibody
    • MGC111005 antibody
    • Ribosomal protein L34 antibody

画像

  • Lane 1 :
    Lane 2 :

    Lane 1 : Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
    Lane 2 : Human cell line HEK 293


    Predicted band size : 13 kDa
  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoplasm. Recommended concentration of ab122255 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122255, at 1/10, staining RPL34 in paraffin embedded Human pancreas tissue by Immunohistochemistry.

参考文献

ab122255 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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